PRODUCTION OF STABLE ISOTOPE-KLABELED CYTOCHROME C3 DERIVED FROM SULFATE REDUCING BACTERIA
PURPOSE:To provide a method for production of stable isotope-labeled cytochrome C3 derived from sulfate reducing bacteria and useful for a biological device and a material for studying biological device. CONSTITUTION:This method for production of stable isotope-labeled cytochrome C3 is carried out b...
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| creator | UEDA RYOHEI OMURA TOMOAKI |
| description | PURPOSE:To provide a method for production of stable isotope-labeled cytochrome C3 derived from sulfate reducing bacteria and useful for a biological device and a material for studying biological device. CONSTITUTION:This method for production of stable isotope-labeled cytochrome C3 is carried out by culturing and growing sulfate reducing bacteria in a chemosynthetic medium containing a nitrogen source composed of plural amino acids in which only a specified amino acid is labeled with a stable isotope under an anaerobic condition and synthesizing cytochrome C3 in the sulfate reducing bacteria, or by culturing and growing sulfate reducing bacteria in a chemosynthetic medium containing a nitrogen source composed of only ammonium chloride which is labeled with a stable isotope under an anaerobic condition and synthesizing cytochrome C3 in the sulfate reducing bacteria. A part of the amino acids constituting the obtained cytochrome C3 or the whole cytochrome C3 can be labeled thereby with the stable isotope with a high level. Especially, this is the first method which can label the whole cytochrome C3 by NH4Cl. In addition, this method can reduce the amount of labeled amino acids to 1/10 in comparison with the conventional method and the cost of expensive stable isotope, therefore, can remarkably be reduced. |
| format | Patent |
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In addition, this method can reduce the amount of labeled amino acids to 1/10 in comparison with the conventional method and the cost of expensive stable isotope, therefore, can remarkably be reduced.</description><edition>6</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1996</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19960130&DB=EPODOC&CC=JP&NR=H0823994A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19960130&DB=EPODOC&CC=JP&NR=H0823994A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>UEDA RYOHEI</creatorcontrib><creatorcontrib>OMURA TOMOAKI</creatorcontrib><title>PRODUCTION OF STABLE ISOTOPE-KLABELED CYTOCHROME C3 DERIVED FROM SULFATE REDUCING BACTERIA</title><description>PURPOSE:To provide a method for production of stable isotope-labeled cytochrome C3 derived from sulfate reducing bacteria and useful for a biological device and a material for studying biological device. 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CONSTITUTION:This method for production of stable isotope-labeled cytochrome C3 is carried out by culturing and growing sulfate reducing bacteria in a chemosynthetic medium containing a nitrogen source composed of plural amino acids in which only a specified amino acid is labeled with a stable isotope under an anaerobic condition and synthesizing cytochrome C3 in the sulfate reducing bacteria, or by culturing and growing sulfate reducing bacteria in a chemosynthetic medium containing a nitrogen source composed of only ammonium chloride which is labeled with a stable isotope under an anaerobic condition and synthesizing cytochrome C3 in the sulfate reducing bacteria. A part of the amino acids constituting the obtained cytochrome C3 or the whole cytochrome C3 can be labeled thereby with the stable isotope with a high level. Especially, this is the first method which can label the whole cytochrome C3 by NH4Cl. In addition, this method can reduce the amount of labeled amino acids to 1/10 in comparison with the conventional method and the cost of expensive stable isotope, therefore, can remarkably be reduced.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | PRODUCTION OF STABLE ISOTOPE-KLABELED CYTOCHROME C3 DERIVED FROM SULFATE REDUCING BACTERIA |
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