METHOD FOR MEASURING AMOUNT OF LIPID AND ASSAY KIT
To provide a simple and accurate method for measuring the amount of lipids contained in a biological sample.SOLUTION: Provided is a method for measuring the amount of lipids contained in a biological sample, comprising: a preparation step of preparing a biological sample immobilized on a substrate;...
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| creator | HARA TSUKASA SANO TAKAHIRO |
| description | To provide a simple and accurate method for measuring the amount of lipids contained in a biological sample.SOLUTION: Provided is a method for measuring the amount of lipids contained in a biological sample, comprising: a preparation step of preparing a biological sample immobilized on a substrate; a first staining step of contacting the biological sample with a first dye for staining lipids to stain the lipids contained in the biological sample; a second staining step of contacting the biological sample with a second dye for staining a cell composition other than the lipids contained in the biological sample to stain the cell composition other than the lipids; a washing step of washing the biological sample after the first staining step and the second staining step to remove an excess of the first dye and the second dye; and an extraction step of extracting the first dye and the second dye from the stained biological sample.SELECTED DRAWING: None
【課題】生体試料に含まれる脂質量を簡便かつ精度よく測定する方法を提供すること。【解決手段】基板に固定化された生体試料を準備する準備工程と、脂質を染色する第一の色素を、前記生体試料に接触させ、前記生体試料に含まれる脂質を染色する第一染色工程と、前記生体試料に含まれる前記脂質以外の細胞組成物を染色する第二の色素を、前記生体試料に接触させ、前記脂質以外の細胞組成物を染色する第二染色工程と、前記第一染色工程及び第二染色工程後の生体試料を洗浄し、余剰の前記第一の色素及び前記第二の色素を除去する洗浄工程と、前記染色された生体試料から前記第一の色素及び前記第二の色素を抽出する抽出工程と、を含む、生体試料に含まれる脂質量の測定方法である。【選択図】なし |
| format | Patent |
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【課題】生体試料に含まれる脂質量を簡便かつ精度よく測定する方法を提供すること。【解決手段】基板に固定化された生体試料を準備する準備工程と、脂質を染色する第一の色素を、前記生体試料に接触させ、前記生体試料に含まれる脂質を染色する第一染色工程と、前記生体試料に含まれる前記脂質以外の細胞組成物を染色する第二の色素を、前記生体試料に接触させ、前記脂質以外の細胞組成物を染色する第二染色工程と、前記第一染色工程及び第二染色工程後の生体試料を洗浄し、余剰の前記第一の色素及び前記第二の色素を除去する洗浄工程と、前記染色された生体試料から前記第一の色素及び前記第二の色素を抽出する抽出工程と、を含む、生体試料に含まれる脂質量の測定方法である。【選択図】なし</description><language>eng ; jpn</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230706&DB=EPODOC&CC=JP&NR=2023095293A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230706&DB=EPODOC&CC=JP&NR=2023095293A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HARA TSUKASA</creatorcontrib><creatorcontrib>SANO TAKAHIRO</creatorcontrib><title>METHOD FOR MEASURING AMOUNT OF LIPID AND ASSAY KIT</title><description>To provide a simple and accurate method for measuring the amount of lipids contained in a biological sample.SOLUTION: Provided is a method for measuring the amount of lipids contained in a biological sample, comprising: a preparation step of preparing a biological sample immobilized on a substrate; a first staining step of contacting the biological sample with a first dye for staining lipids to stain the lipids contained in the biological sample; a second staining step of contacting the biological sample with a second dye for staining a cell composition other than the lipids contained in the biological sample to stain the cell composition other than the lipids; a washing step of washing the biological sample after the first staining step and the second staining step to remove an excess of the first dye and the second dye; and an extraction step of extracting the first dye and the second dye from the stained biological sample.SELECTED DRAWING: None
【課題】生体試料に含まれる脂質量を簡便かつ精度よく測定する方法を提供すること。【解決手段】基板に固定化された生体試料を準備する準備工程と、脂質を染色する第一の色素を、前記生体試料に接触させ、前記生体試料に含まれる脂質を染色する第一染色工程と、前記生体試料に含まれる前記脂質以外の細胞組成物を染色する第二の色素を、前記生体試料に接触させ、前記脂質以外の細胞組成物を染色する第二染色工程と、前記第一染色工程及び第二染色工程後の生体試料を洗浄し、余剰の前記第一の色素及び前記第二の色素を除去する洗浄工程と、前記染色された生体試料から前記第一の色素及び前記第二の色素を抽出する抽出工程と、を含む、生体試料に含まれる脂質量の測定方法である。【選択図】なし</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDDydQ3x8HdRcPMPUvB1dQwODfL0c1dw9PUP9QtR8HdT8PEM8HRRcPQD4uBgx0gFb88QHgbWtMSc4lReKM3NoOTmGuLsoZtakB-fWlyQmJyal1oS7xVgZGBkbGBpamRp7GhMlCIApnQm_Q</recordid><startdate>20230706</startdate><enddate>20230706</enddate><creator>HARA TSUKASA</creator><creator>SANO TAKAHIRO</creator><scope>EVB</scope></search><sort><creationdate>20230706</creationdate><title>METHOD FOR MEASURING AMOUNT OF LIPID AND ASSAY KIT</title><author>HARA TSUKASA ; SANO TAKAHIRO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JP2023095293A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; jpn</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HARA TSUKASA</creatorcontrib><creatorcontrib>SANO TAKAHIRO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HARA TSUKASA</au><au>SANO TAKAHIRO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR MEASURING AMOUNT OF LIPID AND ASSAY KIT</title><date>2023-07-06</date><risdate>2023</risdate><abstract>To provide a simple and accurate method for measuring the amount of lipids contained in a biological sample.SOLUTION: Provided is a method for measuring the amount of lipids contained in a biological sample, comprising: a preparation step of preparing a biological sample immobilized on a substrate; a first staining step of contacting the biological sample with a first dye for staining lipids to stain the lipids contained in the biological sample; a second staining step of contacting the biological sample with a second dye for staining a cell composition other than the lipids contained in the biological sample to stain the cell composition other than the lipids; a washing step of washing the biological sample after the first staining step and the second staining step to remove an excess of the first dye and the second dye; and an extraction step of extracting the first dye and the second dye from the stained biological sample.SELECTED DRAWING: None
【課題】生体試料に含まれる脂質量を簡便かつ精度よく測定する方法を提供すること。【解決手段】基板に固定化された生体試料を準備する準備工程と、脂質を染色する第一の色素を、前記生体試料に接触させ、前記生体試料に含まれる脂質を染色する第一染色工程と、前記生体試料に含まれる前記脂質以外の細胞組成物を染色する第二の色素を、前記生体試料に接触させ、前記脂質以外の細胞組成物を染色する第二染色工程と、前記第一染色工程及び第二染色工程後の生体試料を洗浄し、余剰の前記第一の色素及び前記第二の色素を除去する洗浄工程と、前記染色された生体試料から前記第一の色素及び前記第二の色素を抽出する抽出工程と、を含む、生体試料に含まれる脂質量の測定方法である。【選択図】なし</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | METHOD FOR MEASURING AMOUNT OF LIPID AND ASSAY KIT |
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