CELL CRYOPRESERVATION AGENT, AND CRYOPRESERVATION METHOD OF CELL
To provide cryopreservation liquid that can be transported for a long period of time at cooling temperature of -80°C or higher without using serum, has low cytotoxicity, is available for cryopreservation of a cell aggregate, and a simple and highly efficient cryopreservation method of cells.SOLUTION...
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| creator | KANAI YUUKI ITO ETSUMI OGIWARA NAOTO |
| description | To provide cryopreservation liquid that can be transported for a long period of time at cooling temperature of -80°C or higher without using serum, has low cytotoxicity, is available for cryopreservation of a cell aggregate, and a simple and highly efficient cryopreservation method of cells.SOLUTION: A cell cryopreservation agent contains a bipolar compound and low molecular solvent, but does not contain a natural animal-derived component, and has the osmotic pressure of 500 to 2500 mOsm/kg. Further, the cell cryopreservation agent in which the amount of unfreezable water measured in aqueous solution where the concentration of the bipolar compound is 0.1 to 30.0w/v% is 200 mg or more per 1 g of the bipolar compound.SELECTED DRAWING: Figure 1
【課題】本発明の課題は、血清を使用することなく、-８０℃以上の冷却温度で長時間の輸送が可能であり、細胞毒性が低く、細胞凝集物の凍結にも利用可能な凍結保存液、および簡易で効率の高い細胞の凍結保存方法を提供することである。【解決手段】上記課題は、両極性化合物及び低分子溶剤を含み、天然の動物由来成分を含まない細胞凍結保存剤であって、浸透圧は５００～２５００ｍＯｓｍ／ｋｇであること特徴とする細胞凍結保存剤によって解決することができる。また、両極性化合物は、両極性化合物の濃度が０．１～３０．０ｗ／ｖ％の水溶液において測定した不凍水の量が、両極性化合物１ｇあたり２００ｍｇ以上であることを特徴とする前記細胞凍結保存剤によって解決することができる。【選択図】図１ |
| format | Patent |
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【課題】本発明の課題は、血清を使用することなく、-８０℃以上の冷却温度で長時間の輸送が可能であり、細胞毒性が低く、細胞凝集物の凍結にも利用可能な凍結保存液、および簡易で効率の高い細胞の凍結保存方法を提供することである。【解決手段】上記課題は、両極性化合物及び低分子溶剤を含み、天然の動物由来成分を含まない細胞凍結保存剤であって、浸透圧は５００～２５００ｍＯｓｍ／ｋｇであること特徴とする細胞凍結保存剤によって解決することができる。また、両極性化合物は、両極性化合物の濃度が０．１～３０．０ｗ／ｖ％の水溶液において測定した不凍水の量が、両極性化合物１ｇあたり２００ｍｇ以上であることを特徴とする前記細胞凍結保存剤によって解決することができる。【選択図】図１</description><language>eng ; jpn</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220628&DB=EPODOC&CC=JP&NR=2022095009A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220628&DB=EPODOC&CC=JP&NR=2022095009A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KANAI YUUKI</creatorcontrib><creatorcontrib>ITO ETSUMI</creatorcontrib><creatorcontrib>OGIWARA NAOTO</creatorcontrib><title>CELL CRYOPRESERVATION AGENT, AND CRYOPRESERVATION METHOD OF CELL</title><description>To provide cryopreservation liquid that can be transported for a long period of time at cooling temperature of -80°C or higher without using serum, has low cytotoxicity, is available for cryopreservation of a cell aggregate, and a simple and highly efficient cryopreservation method of cells.SOLUTION: A cell cryopreservation agent contains a bipolar compound and low molecular solvent, but does not contain a natural animal-derived component, and has the osmotic pressure of 500 to 2500 mOsm/kg. Further, the cell cryopreservation agent in which the amount of unfreezable water measured in aqueous solution where the concentration of the bipolar compound is 0.1 to 30.0w/v% is 200 mg or more per 1 g of the bipolar compound.SELECTED DRAWING: Figure 1
【課題】本発明の課題は、血清を使用することなく、-８０℃以上の冷却温度で長時間の輸送が可能であり、細胞毒性が低く、細胞凝集物の凍結にも利用可能な凍結保存液、および簡易で効率の高い細胞の凍結保存方法を提供することである。【解決手段】上記課題は、両極性化合物及び低分子溶剤を含み、天然の動物由来成分を含まない細胞凍結保存剤であって、浸透圧は５００～２５００ｍＯｓｍ／ｋｇであること特徴とする細胞凍結保存剤によって解決することができる。また、両極性化合物は、両極性化合物の濃度が０．１～３０．０ｗ／ｖ％の水溶液において測定した不凍水の量が、両極性化合物１ｇあたり２００ｍｇ以上であることを特徴とする前記細胞凍結保存剤によって解決することができる。【選択図】図１</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHBwdvXxUXAOivQPCHINdg0Kcwzx9PdTcHR39QvRUXD0c8GU83UN8fB3UfB3UwDp5WFgTUvMKU7lhdLcDEpuriHOHrqpBfnxqcUFicmpeakl8V4BRgZGRgaWpgYGlo7GRCkCABbQKzc</recordid><startdate>20220628</startdate><enddate>20220628</enddate><creator>KANAI YUUKI</creator><creator>ITO ETSUMI</creator><creator>OGIWARA NAOTO</creator><scope>EVB</scope></search><sort><creationdate>20220628</creationdate><title>CELL CRYOPRESERVATION AGENT, AND CRYOPRESERVATION METHOD OF CELL</title><author>KANAI YUUKI ; ITO ETSUMI ; OGIWARA NAOTO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JP2022095009A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; jpn</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KANAI YUUKI</creatorcontrib><creatorcontrib>ITO ETSUMI</creatorcontrib><creatorcontrib>OGIWARA NAOTO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KANAI YUUKI</au><au>ITO ETSUMI</au><au>OGIWARA NAOTO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>CELL CRYOPRESERVATION AGENT, AND CRYOPRESERVATION METHOD OF CELL</title><date>2022-06-28</date><risdate>2022</risdate><abstract>To provide cryopreservation liquid that can be transported for a long period of time at cooling temperature of -80°C or higher without using serum, has low cytotoxicity, is available for cryopreservation of a cell aggregate, and a simple and highly efficient cryopreservation method of cells.SOLUTION: A cell cryopreservation agent contains a bipolar compound and low molecular solvent, but does not contain a natural animal-derived component, and has the osmotic pressure of 500 to 2500 mOsm/kg. Further, the cell cryopreservation agent in which the amount of unfreezable water measured in aqueous solution where the concentration of the bipolar compound is 0.1 to 30.0w/v% is 200 mg or more per 1 g of the bipolar compound.SELECTED DRAWING: Figure 1
【課題】本発明の課題は、血清を使用することなく、-８０℃以上の冷却温度で長時間の輸送が可能であり、細胞毒性が低く、細胞凝集物の凍結にも利用可能な凍結保存液、および簡易で効率の高い細胞の凍結保存方法を提供することである。【解決手段】上記課題は、両極性化合物及び低分子溶剤を含み、天然の動物由来成分を含まない細胞凍結保存剤であって、浸透圧は５００～２５００ｍＯｓｍ／ｋｇであること特徴とする細胞凍結保存剤によって解決することができる。また、両極性化合物は、両極性化合物の濃度が０．１～３０．０ｗ／ｖ％の水溶液において測定した不凍水の量が、両極性化合物１ｇあたり２００ｍｇ以上であることを特徴とする前記細胞凍結保存剤によって解決することができる。【選択図】図１</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | CELL CRYOPRESERVATION AGENT, AND CRYOPRESERVATION METHOD OF CELL |
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