METHODS AND KITS FOR PREDICTING DRUG CARDIOTOXICITY
To provide simple methods for predicting the potential for drug-induced prolongation of action potential duration of ventricular muscle.SOLUTION: Provided is a method for predicting the cardiotoxicity of a test substance, which comprises: expressing a protein in which a hERG channel, a transmembrane...
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| description | To provide simple methods for predicting the potential for drug-induced prolongation of action potential duration of ventricular muscle.SOLUTION: Provided is a method for predicting the cardiotoxicity of a test substance, which comprises: expressing a protein in which a hERG channel, a transmembrane area, and one fragment of a full-length reporter protein divided into two is fused, on a cell membrane surface; adding the test substance to a medium to culture the cells; removing the test substance, subsequently adding the other fragment of the full-length reporter protein divided into two to reconstitute the full-length reporter protein on the cells; and measuring the signal from the reconstituted reporter protein.SELECTED DRAWING: Figure 10
【課題】 薬剤により引き起こされる心室筋の活動電位持続時間の延長の可能性を予測するための簡易な方法を提供すること。【解決手段】 ｈERGチャネル、膜貫通領域及び全長レポータータンパク質を２分割した一方のフラグメントを融合させたタンパク質を細胞膜表面上に発現させること、被験物質を培地に添加して前記細胞を培養すること、前記被験物質を除去してから、前記全長レポータータンパク質を２分割した他方のフラグメントを添加して、前記細胞上で前記全長レポータータンパク質を再構成すること、前記再構成されたレポータータンパク質からのシグナルを測定することを含む、被験物質の心毒性を予測する方法。【選択図】 図１０ |
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【課題】 薬剤により引き起こされる心室筋の活動電位持続時間の延長の可能性を予測するための簡易な方法を提供すること。【解決手段】 ｈERGチャネル、膜貫通領域及び全長レポータータンパク質を２分割した一方のフラグメントを融合させたタンパク質を細胞膜表面上に発現させること、被験物質を培地に添加して前記細胞を培養すること、前記被験物質を除去してから、前記全長レポータータンパク質を２分割した他方のフラグメントを添加して、前記細胞上で前記全長レポータータンパク質を再構成すること、前記再構成されたレポータータンパク質からのシグナルを測定することを含む、被験物質の心毒性を予測する方法。【選択図】 図１０</description><language>eng ; jpn</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210301&DB=EPODOC&CC=JP&NR=2021029190A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210301&DB=EPODOC&CC=JP&NR=2021029190A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HORI AYUMI</creatorcontrib><creatorcontrib>YAMAGISHI YUTAKA</creatorcontrib><title>METHODS AND KITS FOR PREDICTING DRUG CARDIOTOXICITY</title><description>To provide simple methods for predicting the potential for drug-induced prolongation of action potential duration of ventricular muscle.SOLUTION: Provided is a method for predicting the cardiotoxicity of a test substance, which comprises: expressing a protein in which a hERG channel, a transmembrane area, and one fragment of a full-length reporter protein divided into two is fused, on a cell membrane surface; adding the test substance to a medium to culture the cells; removing the test substance, subsequently adding the other fragment of the full-length reporter protein divided into two to reconstitute the full-length reporter protein on the cells; and measuring the signal from the reconstituted reporter protein.SELECTED DRAWING: Figure 10
【課題】 薬剤により引き起こされる心室筋の活動電位持続時間の延長の可能性を予測するための簡易な方法を提供すること。【解決手段】 ｈERGチャネル、膜貫通領域及び全長レポータータンパク質を２分割した一方のフラグメントを融合させたタンパク質を細胞膜表面上に発現させること、被験物質を培地に添加して前記細胞を培養すること、前記被験物質を除去してから、前記全長レポータータンパク質を２分割した他方のフラグメントを添加して、前記細胞上で前記全長レポータータンパク質を再構成すること、前記再構成されたレポータータンパク質からのシグナルを測定することを含む、被験物質の心毒性を予測する方法。【選択図】 図１０</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDD2dQ3x8HcJVnD0c1Hw9gwJVnDzD1IICHJ18XQO8fRzV3AJCnVXcHYMcvH0D_GP8HT2DInkYWBNS8wpTuWF0twMSm6uIc4euqkF-fGpxQWJyal5qSXxXgFGBkaGBkaWhpYGjsZEKQIA7sQnlQ</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>HORI AYUMI</creator><creator>YAMAGISHI YUTAKA</creator><scope>EVB</scope></search><sort><creationdate>20210301</creationdate><title>METHODS AND KITS FOR PREDICTING DRUG CARDIOTOXICITY</title><author>HORI AYUMI ; YAMAGISHI YUTAKA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JP2021029190A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; jpn</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HORI AYUMI</creatorcontrib><creatorcontrib>YAMAGISHI YUTAKA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HORI AYUMI</au><au>YAMAGISHI YUTAKA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHODS AND KITS FOR PREDICTING DRUG CARDIOTOXICITY</title><date>2021-03-01</date><risdate>2021</risdate><abstract>To provide simple methods for predicting the potential for drug-induced prolongation of action potential duration of ventricular muscle.SOLUTION: Provided is a method for predicting the cardiotoxicity of a test substance, which comprises: expressing a protein in which a hERG channel, a transmembrane area, and one fragment of a full-length reporter protein divided into two is fused, on a cell membrane surface; adding the test substance to a medium to culture the cells; removing the test substance, subsequently adding the other fragment of the full-length reporter protein divided into two to reconstitute the full-length reporter protein on the cells; and measuring the signal from the reconstituted reporter protein.SELECTED DRAWING: Figure 10
【課題】 薬剤により引き起こされる心室筋の活動電位持続時間の延長の可能性を予測するための簡易な方法を提供すること。【解決手段】 ｈERGチャネル、膜貫通領域及び全長レポータータンパク質を２分割した一方のフラグメントを融合させたタンパク質を細胞膜表面上に発現させること、被験物質を培地に添加して前記細胞を培養すること、前記被験物質を除去してから、前記全長レポータータンパク質を２分割した他方のフラグメントを添加して、前記細胞上で前記全長レポータータンパク質を再構成すること、前記再構成されたレポータータンパク質からのシグナルを測定することを含む、被験物質の心毒性を予測する方法。【選択図】 図１０</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHODS AND KITS FOR PREDICTING DRUG CARDIOTOXICITY |
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