FIBRINOGEN QUANTIFICATION METHOD

To provide a fibrinogen quantification method capable of accurate fibrinogen quantification without requiring preparing reagents or dilute samples, applicable to perinatal and perioperative use.SOLUTION: The fibrinogen quantification method includes the steps of: after adding the sample to the fibrinogen quantitative drying reagent containing magnetic particles, moving magnetic particles in the reagent to monitor movement signals of the magnetic particles; and calculating multiple magnetic particle motion signal ratios at regular time intervals. Defining an arbitrary point in the section where the ratio is maintained within a certain range for a certain period as a starting point and a point that is 5-50% attenuated with respect to the peak value of the magnetic particle motion signal after the starting point as the endpoint, the solidification time is the time from the start point to the endpoint. With this, accurate fibrinogen quantification can be performed even when undiluted whole blood is used as the measurement sample.SELECTED DRAWING: Figure 4 【課題】周産期および周術期での使用に耐えうる、試薬の調製や検体の希釈操作を必要とせず、且つ、正確なフィブリノゲン定量が可能なフィブリノゲン定量方法の開発。【解決手段】磁性粒子を含有したフィブリノゲン定量乾燥試薬に検体を添加後、試薬中の磁性粒子を運動させ、磁性粒子運動シグナルをモニターするとともに、一定の時間間隔の磁性粒子運動シグナル比を複数算出し、その比が一定の範囲内で一定時間保たれた区間の任意の点を起点とし、起点以降の磁性粒子運動シグナルのピーク値に対して5〜50％減衰した点を終点とし、起点から終点までの時間を凝固時間とすることを特徴とするフィブリノゲン定量方法を適用することにより、測定試料を無希釈全血とした場合でも、正確なフィブリノゲン定量を可能とする。【選択図】図４