LIQUID CHROMATOGRAPH METHOD AND LIQUID CHROMATOGRAPHY ASSISTANT DEVICE

PROBLEM TO BE SOLVED: To provide a method that can predict the result of liquid chromatography precisely for a wide range of solvent mixture ratios based on the result of thin-layer chromatography.SOLUTION: The present invention relates to a liquid chromatography method for separating two or more di...

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1. Verfasser: OKURA KIHACHIRO
Format: Patent
Sprache:eng ; jpn
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Zusammenfassung:PROBLEM TO BE SOLVED: To provide a method that can predict the result of liquid chromatography precisely for a wide range of solvent mixture ratios based on the result of thin-layer chromatography.SOLUTION: The present invention relates to a liquid chromatography method for separating two or more different types of compounds of a mixture, the method including: step (1) of conducting thin-layer chromatography or column chromatography on two or more solvent mixtures with different mixture ratios or a single solvent; step (2) of creating a relational expression of the solvent mixture ratio and the elution degree of each compound in a sample based on the result of step (1); and step (3) of determining an optimum condition based on the expression and conducting liquid chromatography.SELECTED DRAWING: Figure 2 【課題】薄層クロマトグラフィの結果を利用して液体クロマトグラフィの結果を幅広い溶媒混合比に対して高精度で予測する方法を提供する。【解決手段】2種類以上の化合物の混合物を分離するための液体クロマトグラフィ方法であって、混合比が異なる2種以上の混合溶媒又は単独溶媒において薄層クロマトグラフィ又はカラムクロマトグラフィを行う工程(1)、工程(1)の結果に基づいてサンプル中の化合物それぞれについて溶媒の混合比率と溶出度との関係式を作成する工程(2)、及び、関係式に基づいて最適条件を決定して液体クロマトグラフィを行う工程(3)を有する。【選択図】図2