METHOD FOR DETECTING CYCLIC AMP

PROBLEM TO BE SOLVED: To provide a method for detecting cAMP in cells without expression of cAMP binding protein in cells.SOLUTION: Provided is a cAMP detection method for detecting or quantifying cAMP, which comprises: (a) a step of preparing a cell 1 expressing connexin molecules or innexin molecu...

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Hauptverfasser: URYU YUKITSUGU, SHIMONO TAKESHI, NAGAI KENJI
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creator URYU YUKITSUGU
SHIMONO TAKESHI
NAGAI KENJI
description PROBLEM TO BE SOLVED: To provide a method for detecting cAMP in cells without expression of cAMP binding protein in cells.SOLUTION: Provided is a cAMP detection method for detecting or quantifying cAMP, which comprises: (a) a step of preparing a cell 1 expressing connexin molecules or innexin molecules and a liposome 2 presenting connexin molecules or innexin molecules where the liposome 2 contains cAMP detection reagent inside thereof; (b) a step of forming a passageway 4 between the cell 1 and the liposome 2 mediated by connexin or innexin molecules; (c) a step in which cAMP produced in the cell 1 moves into the liposome 2 and binds to the cAMP detection reagent and the cAMP detection reagent turns into cAMP-bound reagent 6; (d) a step of detecting the change in the property of the detection reagent due to the shift from the cAMP detection reagent 3 to the cAMP-bound reagent 6. 【課題】細胞内にcAMP結合型タンパク質を発現させることなく、細胞内のcAMPを検出する方法を提供する。【解決手段】cAMP検出方法は、cAMPを検知または定量する方法であって、(a)コネキシン分子もしくはイネキシン分子を発現する細胞1と、コネキシン分子もしくはイネキシン分子を提示するリポソーム2であって、内部にcAMP検出試薬を有するリポソーム2とを準備する工程と、(b)細胞1とリポソーム2との間に、コネキシン分子もしくはイネキシン分子を介した連絡通路4を形成させる工程と、(c)細胞1によって産生されたcAMPは連絡通路4を通してリポソーム2に移動し、リポソーム2の中でcAMPがcAMP検出試薬と結合し、cAMP検出試薬3がcAMP結合状態試薬6に変化する工程と、(d)cAMP検出試薬3からcAMP結合状態試薬6への変化にともなう、検出試薬の性質の変化を検出する工程とを包含する。【選択図】図1
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SHIMONO TAKESHI ; NAGAI KENJI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JP2015186463A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; jpn</language><creationdate>2015</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>URYU YUKITSUGU</creatorcontrib><creatorcontrib>SHIMONO TAKESHI</creatorcontrib><creatorcontrib>NAGAI KENJI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>URYU YUKITSUGU</au><au>SHIMONO TAKESHI</au><au>NAGAI KENJI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR DETECTING CYCLIC AMP</title><date>2015-10-29</date><risdate>2015</risdate><abstract>PROBLEM TO BE SOLVED: To provide a method for detecting cAMP in cells without expression of cAMP binding protein in cells.SOLUTION: Provided is a cAMP detection method for detecting or quantifying cAMP, which comprises: (a) a step of preparing a cell 1 expressing connexin molecules or innexin molecules and a liposome 2 presenting connexin molecules or innexin molecules where the liposome 2 contains cAMP detection reagent inside thereof; (b) a step of forming a passageway 4 between the cell 1 and the liposome 2 mediated by connexin or innexin molecules; (c) a step in which cAMP produced in the cell 1 moves into the liposome 2 and binds to the cAMP detection reagent and the cAMP detection reagent turns into cAMP-bound reagent 6; (d) a step of detecting the change in the property of the detection reagent due to the shift from the cAMP detection reagent 3 to the cAMP-bound reagent 6. 【課題】細胞内にcAMP結合型タンパク質を発現させることなく、細胞内のcAMPを検出する方法を提供する。【解決手段】cAMP検出方法は、cAMPを検知または定量する方法であって、(a)コネキシン分子もしくはイネキシン分子を発現する細胞1と、コネキシン分子もしくはイネキシン分子を提示するリポソーム2であって、内部にcAMP検出試薬を有するリポソーム2とを準備する工程と、(b)細胞1とリポソーム2との間に、コネキシン分子もしくはイネキシン分子を介した連絡通路4を形成させる工程と、(c)細胞1によって産生されたcAMPは連絡通路4を通してリポソーム2に移動し、リポソーム2の中でcAMPがcAMP検出試薬と結合し、cAMP検出試薬3がcAMP結合状態試薬6に変化する工程と、(d)cAMP検出試薬3からcAMP結合状態試薬6への変化にともなう、検出試薬の性質の変化を検出する工程とを包含する。【選択図】図1</abstract><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title METHOD FOR DETECTING CYCLIC AMP
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