METHOD FOR DETECTING CYCLIC AMP
PROBLEM TO BE SOLVED: To provide a method for detecting cAMP in cells without expression of cAMP binding protein in cells.SOLUTION: Provided is a cAMP detection method for detecting or quantifying cAMP, which comprises: (a) a step of preparing a cell 1 expressing connexin molecules or innexin molecu...
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description | PROBLEM TO BE SOLVED: To provide a method for detecting cAMP in cells without expression of cAMP binding protein in cells.SOLUTION: Provided is a cAMP detection method for detecting or quantifying cAMP, which comprises: (a) a step of preparing a cell 1 expressing connexin molecules or innexin molecules and a liposome 2 presenting connexin molecules or innexin molecules where the liposome 2 contains cAMP detection reagent inside thereof; (b) a step of forming a passageway 4 between the cell 1 and the liposome 2 mediated by connexin or innexin molecules; (c) a step in which cAMP produced in the cell 1 moves into the liposome 2 and binds to the cAMP detection reagent and the cAMP detection reagent turns into cAMP-bound reagent 6; (d) a step of detecting the change in the property of the detection reagent due to the shift from the cAMP detection reagent 3 to the cAMP-bound reagent 6.
【課題】細胞内にcAMP結合型タンパク質を発現させることなく、細胞内のcAMPを検出する方法を提供する。【解決手段】cAMP検出方法は、cAMPを検知または定量する方法であって、(a)コネキシン分子もしくはイネキシン分子を発現する細胞1と、コネキシン分子もしくはイネキシン分子を提示するリポソーム2であって、内部にcAMP検出試薬を有するリポソーム2とを準備する工程と、(b)細胞1とリポソーム2との間に、コネキシン分子もしくはイネキシン分子を介した連絡通路4を形成させる工程と、(c)細胞1によって産生されたcAMPは連絡通路4を通してリポソーム2に移動し、リポソーム2の中でcAMPがcAMP検出試薬と結合し、cAMP検出試薬3がcAMP結合状態試薬6に変化する工程と、(d)cAMP検出試薬3からcAMP結合状態試薬6への変化にともなう、検出試薬の性質の変化を検出する工程とを包含する。【選択図】図1 |
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【課題】細胞内にcAMP結合型タンパク質を発現させることなく、細胞内のcAMPを検出する方法を提供する。【解決手段】cAMP検出方法は、cAMPを検知または定量する方法であって、(a)コネキシン分子もしくはイネキシン分子を発現する細胞1と、コネキシン分子もしくはイネキシン分子を提示するリポソーム2であって、内部にcAMP検出試薬を有するリポソーム2とを準備する工程と、(b)細胞1とリポソーム2との間に、コネキシン分子もしくはイネキシン分子を介した連絡通路4を形成させる工程と、(c)細胞1によって産生されたcAMPは連絡通路4を通してリポソーム2に移動し、リポソーム2の中でcAMPがcAMP検出試薬と結合し、cAMP検出試薬3がcAMP結合状態試薬6に変化する工程と、(d)cAMP検出試薬3からcAMP結合状態試薬6への変化にともなう、検出試薬の性質の変化を検出する工程とを包含する。【選択図】図1</description><language>eng ; jpn</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2015</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20151029&DB=EPODOC&CC=JP&NR=2015186463A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20151029&DB=EPODOC&CC=JP&NR=2015186463A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>URYU YUKITSUGU</creatorcontrib><creatorcontrib>SHIMONO TAKESHI</creatorcontrib><creatorcontrib>NAGAI KENJI</creatorcontrib><title>METHOD FOR DETECTING CYCLIC AMP</title><description>PROBLEM TO BE SOLVED: To provide a method for detecting cAMP in cells without expression of cAMP binding protein in cells.SOLUTION: Provided is a cAMP detection method for detecting or quantifying cAMP, which comprises: (a) a step of preparing a cell 1 expressing connexin molecules or innexin molecules and a liposome 2 presenting connexin molecules or innexin molecules where the liposome 2 contains cAMP detection reagent inside thereof; (b) a step of forming a passageway 4 between the cell 1 and the liposome 2 mediated by connexin or innexin molecules; (c) a step in which cAMP produced in the cell 1 moves into the liposome 2 and binds to the cAMP detection reagent and the cAMP detection reagent turns into cAMP-bound reagent 6; (d) a step of detecting the change in the property of the detection reagent due to the shift from the cAMP detection reagent 3 to the cAMP-bound reagent 6.
【課題】細胞内にcAMP結合型タンパク質を発現させることなく、細胞内のcAMPを検出する方法を提供する。【解決手段】cAMP検出方法は、cAMPを検知または定量する方法であって、(a)コネキシン分子もしくはイネキシン分子を発現する細胞1と、コネキシン分子もしくはイネキシン分子を提示するリポソーム2であって、内部にcAMP検出試薬を有するリポソーム2とを準備する工程と、(b)細胞1とリポソーム2との間に、コネキシン分子もしくはイネキシン分子を介した連絡通路4を形成させる工程と、(c)細胞1によって産生されたcAMPは連絡通路4を通してリポソーム2に移動し、リポソーム2の中でcAMPがcAMP検出試薬と結合し、cAMP検出試薬3がcAMP結合状態試薬6に変化する工程と、(d)cAMP検出試薬3からcAMP結合状態試薬6への変化にともなう、検出試薬の性質の変化を検出する工程とを包含する。【選択図】図1</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2015</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZJD3dQ3x8HdRcPMPUnBxDXF1DvH0c1dwjnT28XRWcPQN4GFgTUvMKU7lhdLcDEpuriHOHrqpBfnxqcUFicmpeakl8V4BRgaGpoYWZiZmxo7GRCkCAPx1IdY</recordid><startdate>20151029</startdate><enddate>20151029</enddate><creator>URYU YUKITSUGU</creator><creator>SHIMONO TAKESHI</creator><creator>NAGAI KENJI</creator><scope>EVB</scope></search><sort><creationdate>20151029</creationdate><title>METHOD FOR DETECTING CYCLIC AMP</title><author>URYU YUKITSUGU ; SHIMONO TAKESHI ; NAGAI KENJI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JP2015186463A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; jpn</language><creationdate>2015</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>URYU YUKITSUGU</creatorcontrib><creatorcontrib>SHIMONO TAKESHI</creatorcontrib><creatorcontrib>NAGAI KENJI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>URYU YUKITSUGU</au><au>SHIMONO TAKESHI</au><au>NAGAI KENJI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR DETECTING CYCLIC AMP</title><date>2015-10-29</date><risdate>2015</risdate><abstract>PROBLEM TO BE SOLVED: To provide a method for detecting cAMP in cells without expression of cAMP binding protein in cells.SOLUTION: Provided is a cAMP detection method for detecting or quantifying cAMP, which comprises: (a) a step of preparing a cell 1 expressing connexin molecules or innexin molecules and a liposome 2 presenting connexin molecules or innexin molecules where the liposome 2 contains cAMP detection reagent inside thereof; (b) a step of forming a passageway 4 between the cell 1 and the liposome 2 mediated by connexin or innexin molecules; (c) a step in which cAMP produced in the cell 1 moves into the liposome 2 and binds to the cAMP detection reagent and the cAMP detection reagent turns into cAMP-bound reagent 6; (d) a step of detecting the change in the property of the detection reagent due to the shift from the cAMP detection reagent 3 to the cAMP-bound reagent 6.
【課題】細胞内にcAMP結合型タンパク質を発現させることなく、細胞内のcAMPを検出する方法を提供する。【解決手段】cAMP検出方法は、cAMPを検知または定量する方法であって、(a)コネキシン分子もしくはイネキシン分子を発現する細胞1と、コネキシン分子もしくはイネキシン分子を提示するリポソーム2であって、内部にcAMP検出試薬を有するリポソーム2とを準備する工程と、(b)細胞1とリポソーム2との間に、コネキシン分子もしくはイネキシン分子を介した連絡通路4を形成させる工程と、(c)細胞1によって産生されたcAMPは連絡通路4を通してリポソーム2に移動し、リポソーム2の中でcAMPがcAMP検出試薬と結合し、cAMP検出試薬3がcAMP結合状態試薬6に変化する工程と、(d)cAMP検出試薬3からcAMP結合状態試薬6への変化にともなう、検出試薬の性質の変化を検出する工程とを包含する。【選択図】図1</abstract><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
title | METHOD FOR DETECTING CYCLIC AMP |
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