MOLECULAR DIAGNOSTICS SYSTEM AND METHOD
PROBLEM TO BE SOLVED: To provide an automated device (integrated cartridge) and method for extraction of nucleic acids obtained from cells, amplification of nucleic acids, and detection of nucleic acids.SOLUTION: A device comprises: a housing; an entry port 413 for accepting a sample suspected of co...
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| creator | MACLEAD JASON ANDREW COLLIER GORDON BRUCE NEMETH ATTILA CSABA WOOD JOHN ALLISTER DICKE WILLIAM CHARLES MILLER CARY JAMES |
| description | PROBLEM TO BE SOLVED: To provide an automated device (integrated cartridge) and method for extraction of nucleic acids obtained from cells, amplification of nucleic acids, and detection of nucleic acids.SOLUTION: A device comprises: a housing; an entry port 413 for accepting a sample suspected of containing a target nucleic acid; first chambers 408, 409 containing a reagent for extracting the target nucleic acid; and a first conduit permitting passage of extracted nucleic acid. The housing contains an amplification reagent that is capable of incorporating a detectable label into an amplified nucleic acid target. An amplification chamber 410 has means for controlling amplification conditions, and includes means for moving the amplified target to a sensing region to permit binding to the capture oligonucleotide. The second conduit is attached to a holding chamber which contains a fluid capable of substantially displacing an uncaptured amplified target from the sensing region and permits sensing of the detectable label retained in the sensing region.
【課題】細胞から得られる核酸の抽出、核酸の増幅及び核酸の検出のための自動化された装置（１体化されたカートリッジ）及び方法の提供。【解決手段】収容部と、標的核酸を含有すると疑われる試料を受容するための入口ポート４１３と、前記標的核酸を抽出するための試薬を含有する第一のチャンバー４０８、４０９と、抽出された核酸の通過を可能とする第一の伝導路とを含み、前記収容部は、増幅された核酸標的中に検出可能な標識を取り込ませることができる増幅試薬を含有し、増幅チャンバー４１０は、増幅条件を調節するため手段を有し、前記増幅された標的を検知領域へ移動させて、前記捕捉オリゴヌクレオチドとの結合を可能とする手段を含有し、前記第二の伝導路は、検知領域から捕捉されていない増幅された標的を実質的に排除できる流体を含有し、前記検知領域中に保持された前記検出可能な標識の検知を可能とする保持チャンバーへ取り付けられている装置。【選択図】図１８ |
| format | Patent |
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【課題】細胞から得られる核酸の抽出、核酸の増幅及び核酸の検出のための自動化された装置（１体化されたカートリッジ）及び方法の提供。【解決手段】収容部と、標的核酸を含有すると疑われる試料を受容するための入口ポート４１３と、前記標的核酸を抽出するための試薬を含有する第一のチャンバー４０８、４０９と、抽出された核酸の通過を可能とする第一の伝導路とを含み、前記収容部は、増幅された核酸標的中に検出可能な標識を取り込ませることができる増幅試薬を含有し、増幅チャンバー４１０は、増幅条件を調節するため手段を有し、前記増幅された標的を検知領域へ移動させて、前記捕捉オリゴヌクレオチドとの結合を可能とする手段を含有し、前記第二の伝導路は、検知領域から捕捉されていない増幅された標的を実質的に排除できる流体を含有し、前記検知領域中に保持された前記検出可能な標識の検知を可能とする保持チャンバーへ取り付けられている装置。【選択図】図１８</description><language>eng ; jpn</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2015</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150402&DB=EPODOC&CC=JP&NR=2015061526A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150402&DB=EPODOC&CC=JP&NR=2015061526A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MACLEAD JASON ANDREW</creatorcontrib><creatorcontrib>COLLIER GORDON BRUCE</creatorcontrib><creatorcontrib>NEMETH ATTILA CSABA</creatorcontrib><creatorcontrib>WOOD JOHN ALLISTER</creatorcontrib><creatorcontrib>DICKE WILLIAM CHARLES</creatorcontrib><creatorcontrib>MILLER CARY JAMES</creatorcontrib><title>MOLECULAR DIAGNOSTICS SYSTEM AND METHOD</title><description>PROBLEM TO BE SOLVED: To provide an automated device (integrated cartridge) and method for extraction of nucleic acids obtained from cells, amplification of nucleic acids, and detection of nucleic acids.SOLUTION: A device comprises: a housing; an entry port 413 for accepting a sample suspected of containing a target nucleic acid; first chambers 408, 409 containing a reagent for extracting the target nucleic acid; and a first conduit permitting passage of extracted nucleic acid. The housing contains an amplification reagent that is capable of incorporating a detectable label into an amplified nucleic acid target. An amplification chamber 410 has means for controlling amplification conditions, and includes means for moving the amplified target to a sensing region to permit binding to the capture oligonucleotide. The second conduit is attached to a holding chamber which contains a fluid capable of substantially displacing an uncaptured amplified target from the sensing region and permits sensing of the detectable label retained in the sensing region.
【課題】細胞から得られる核酸の抽出、核酸の増幅及び核酸の検出のための自動化された装置（１体化されたカートリッジ）及び方法の提供。【解決手段】収容部と、標的核酸を含有すると疑われる試料を受容するための入口ポート４１３と、前記標的核酸を抽出するための試薬を含有する第一のチャンバー４０８、４０９と、抽出された核酸の通過を可能とする第一の伝導路とを含み、前記収容部は、増幅された核酸標的中に検出可能な標識を取り込ませることができる増幅試薬を含有し、増幅チャンバー４１０は、増幅条件を調節するため手段を有し、前記増幅された標的を検知領域へ移動させて、前記捕捉オリゴヌクレオチドとの結合を可能とする手段を含有し、前記第二の伝導路は、検知領域から捕捉されていない増幅された標的を実質的に排除できる流体を含有し、前記検知領域中に保持された前記検出可能な標識の検知を可能とする保持チャンバーへ取り付けられている装置。【選択図】図１８</description><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2015</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZFD39fdxdQ71cQxScPF0dPfzDw7xdA5WCI4MDnH1VXD0c1HwdQ3x8HfhYWBNS8wpTuWF0twMSm6uIc4euqkF-fGpxQWJyal5qSXxXgFGBoamBmaGpkZmjsZEKQIANiskTw</recordid><startdate>20150402</startdate><enddate>20150402</enddate><creator>MACLEAD JASON ANDREW</creator><creator>COLLIER GORDON BRUCE</creator><creator>NEMETH ATTILA CSABA</creator><creator>WOOD JOHN ALLISTER</creator><creator>DICKE WILLIAM CHARLES</creator><creator>MILLER CARY JAMES</creator><scope>EVB</scope></search><sort><creationdate>20150402</creationdate><title>MOLECULAR DIAGNOSTICS SYSTEM AND METHOD</title><author>MACLEAD JASON ANDREW ; COLLIER GORDON BRUCE ; NEMETH ATTILA CSABA ; WOOD JOHN ALLISTER ; DICKE WILLIAM CHARLES ; MILLER CARY JAMES</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JP2015061526A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; jpn</language><creationdate>2015</creationdate><topic>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>MACLEAD JASON ANDREW</creatorcontrib><creatorcontrib>COLLIER GORDON BRUCE</creatorcontrib><creatorcontrib>NEMETH ATTILA CSABA</creatorcontrib><creatorcontrib>WOOD JOHN ALLISTER</creatorcontrib><creatorcontrib>DICKE WILLIAM CHARLES</creatorcontrib><creatorcontrib>MILLER CARY JAMES</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MACLEAD JASON ANDREW</au><au>COLLIER GORDON BRUCE</au><au>NEMETH ATTILA CSABA</au><au>WOOD JOHN ALLISTER</au><au>DICKE WILLIAM CHARLES</au><au>MILLER CARY JAMES</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>MOLECULAR DIAGNOSTICS SYSTEM AND METHOD</title><date>2015-04-02</date><risdate>2015</risdate><abstract>PROBLEM TO BE SOLVED: To provide an automated device (integrated cartridge) and method for extraction of nucleic acids obtained from cells, amplification of nucleic acids, and detection of nucleic acids.SOLUTION: A device comprises: a housing; an entry port 413 for accepting a sample suspected of containing a target nucleic acid; first chambers 408, 409 containing a reagent for extracting the target nucleic acid; and a first conduit permitting passage of extracted nucleic acid. The housing contains an amplification reagent that is capable of incorporating a detectable label into an amplified nucleic acid target. An amplification chamber 410 has means for controlling amplification conditions, and includes means for moving the amplified target to a sensing region to permit binding to the capture oligonucleotide. The second conduit is attached to a holding chamber which contains a fluid capable of substantially displacing an uncaptured amplified target from the sensing region and permits sensing of the detectable label retained in the sensing region.
【課題】細胞から得られる核酸の抽出、核酸の増幅及び核酸の検出のための自動化された装置（１体化されたカートリッジ）及び方法の提供。【解決手段】収容部と、標的核酸を含有すると疑われる試料を受容するための入口ポート４１３と、前記標的核酸を抽出するための試薬を含有する第一のチャンバー４０８、４０９と、抽出された核酸の通過を可能とする第一の伝導路とを含み、前記収容部は、増幅された核酸標的中に検出可能な標識を取り込ませることができる増幅試薬を含有し、増幅チャンバー４１０は、増幅条件を調節するため手段を有し、前記増幅された標的を検知領域へ移動させて、前記捕捉オリゴヌクレオチドとの結合を可能とする手段を含有し、前記第二の伝導路は、検知領域から捕捉されていない増幅された標的を実質的に排除できる流体を含有し、前記検知領域中に保持された前記検出可能な標識の検知を可能とする保持チャンバーへ取り付けられている装置。【選択図】図１８</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | MOLECULAR DIAGNOSTICS SYSTEM AND METHOD |
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