REACTION METHOD FOR ORGANIC MOLECULE AND AGENT

PROBLEM TO BE SOLVED: To provide a method by which a plurality of organic molecules and an interaction of a plurality of agents can be set as an independent reaction units in an aqueous solution by using an organic membrane, and thereby the plurality of organic molecules and the interaction of the p...

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Hauptverfasser: SATO YUICHI, SHIMIZU KAYO
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creator SATO YUICHI
SHIMIZU KAYO
description PROBLEM TO BE SOLVED: To provide a method by which a plurality of organic molecules and an interaction of a plurality of agents can be set as an independent reaction units in an aqueous solution by using an organic membrane, and thereby the plurality of organic molecules and the interaction of the plurality of agents are simultaneously detected, and which solves deterioration of detection sensitivity due to a nonspecific binding event and realizes a further cost reduction.SOLUTION: In a reaction method for an organic molecule and an agent, a plurality of organic molecules are immobilized on an organic membrane substrate, the organic membrane substrate experiences capillary saturation with a buffer solution after blocking treatment, and then a spot pin which collects and holds the agent at the end is overlapped with and brought into contact with a position of the organic molecule immobilized on the organic membrane substrate, a liquid pool is generated by oozing the buffer solution from the organic membrane substrate to a part in contact with the end of the spot pin, and the organic molecule immobilized on the organic membrane substrate in the above state and the agent overlapped and brought into contact with the spot pin are subjected to a local reaction in the buffer solution.
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title REACTION METHOD FOR ORGANIC MOLECULE AND AGENT
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