METHOD FOR AMPLIFYING AND DETECTING TARGET NUCLEIC ACID

PROBLEM TO BE SOLVED: To provide a method for amplifying and detecting a target nucleic acid.SOLUTION: The method includes contacting a sample suspected of containing the target nucleic acid with two primers and a thermostable DNA polymerase that are substantially complementary to the target nucleic...

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Bibliographische Detailangaben
Hauptverfasser:	MARCIA LYNN KRAMER, BACKUS JOHN WESLEY, FALVO JOSEPH
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for amplifying and detecting a target nucleic acid.SOLUTION: The method includes contacting a sample suspected of containing the target nucleic acid with two primers and a thermostable DNA polymerase that are substantially complementary to the target nucleic acid, under conditions such that the target nucleic acid is amplified. The amplified target nucleic acids are then denatured to form single stranded nucleic acids. Following amplification, the sample is subject to a pre-detection incubation step. The sample is incubated for between 1 second and 30 minutes at between 95°C and 120°C to inactivate the polymerization agent. Finally, the presence or absence of the amplified target nucleic acids is determined.