APPARATUS FOR PREPARING PROTEIN CRYSTAL AND METHOD FOR PREPARING PROTEIN CRYSTAL

PROBLEM TO BE SOLVED: To provide a protein crystal-preparing apparatus and a protein crystal-preparing method, in which protein crystals can be prepared by efficiently attaining protein crystallization conditions having high probability. SOLUTION: The method for preparing the protein crystals compri...

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Hauptverfasser: MOGI ITSURO, OKUTSU TETSUO
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OKUTSU TETSUO
description PROBLEM TO BE SOLVED: To provide a protein crystal-preparing apparatus and a protein crystal-preparing method, in which protein crystals can be prepared by efficiently attaining protein crystallization conditions having high probability. SOLUTION: The method for preparing the protein crystals comprises irradiating a protein solution in a well for screening among a plurality of wells installed in a container with light for preparation of protein crystals, measuring turbidity of the protein solution after light for preparation of protein crystals was irradiated, obtaining irradiation condition of light giving turbidity in which crystallization is expected in highest probability based on detection result of turbidity detection, setting this irradiation conduction as crystallization condition of light for preparation of crystals, then irradiating the well for crystal preparation with light for preparation of crystals based on set crystallization condition to produce crystal nuclei in the protein solution, growing the crystal nuclei, to carry out the preparation of protein crystals. COPYRIGHT: (C)2009,JPO&INPIT
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SOLUTION: The method for preparing the protein crystals comprises irradiating a protein solution in a well for screening among a plurality of wells installed in a container with light for preparation of protein crystals, measuring turbidity of the protein solution after light for preparation of protein crystals was irradiated, obtaining irradiation condition of light giving turbidity in which crystallization is expected in highest probability based on detection result of turbidity detection, setting this irradiation conduction as crystallization condition of light for preparation of crystals, then irradiating the well for crystal preparation with light for preparation of crystals based on set crystallization condition to produce crystal nuclei in the protein solution, growing the crystal nuclei, to carry out the preparation of protein crystals. 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subjects AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUSPOLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE
APPARATUS THEREFOR
CHEMISTRY
CRYSTAL GROWTH
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITHDEFINED STRUCTURE
REFINING BY ZONE-MELTING OF MATERIAL
SEPARATION
SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITHDEFINED STRUCTURE
SINGLE-CRYSTAL-GROWTH
TESTING
TRANSPORTING
UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL ORUNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL
title APPARATUS FOR PREPARING PROTEIN CRYSTAL AND METHOD FOR PREPARING PROTEIN CRYSTAL
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