METHOD FOR PREPARING ARTIFICIAL POSITIVE CONTROL DNA GROUP FOR MULTIPLEX PCR
PROBLEM TO BE SOLVED: To provide a method for preparing an artificial positive control DNA for multiplex PCR confirming, with a single reaction system, misjudgement prevention, the definite discrimination of respective positive control bands and the reactivity of all primer couples. SOLUTION: Each o...
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| creator | SUZUKI YASUSHI ASANO SHIZUKA |
| description | PROBLEM TO BE SOLVED: To provide a method for preparing an artificial positive control DNA for multiplex PCR confirming, with a single reaction system, misjudgement prevention, the definite discrimination of respective positive control bands and the reactivity of all primer couples. SOLUTION: Each of the primer couples are prepared by the following steps(a) to (c): (a) In an available DNA with a known sequence, a domain having any number of bases between 50 and 2,000 is selected, (b) the primer couple is synthesized, which is composed of a forward primer wherein 10-30 bases on the 5' terminal of the domain selected in the step(a) are joined to the 3' side of the forward primer for the multiplex PCR and a reverse primer wherein a complementary sequence of 10-30 bases on the 3' terminal of the domain selected in the step(a) is joined to the 3' side of the reverse primer, and (c) a PCR reaction is conducted using the primer couple synthesized in the step(b). COPYRIGHT: (C)2008,JPO&INPIT |
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SOLUTION: Each of the primer couples are prepared by the following steps(a) to (c): (a) In an available DNA with a known sequence, a domain having any number of bases between 50 and 2,000 is selected, (b) the primer couple is synthesized, which is composed of a forward primer wherein 10-30 bases on the 5' terminal of the domain selected in the step(a) are joined to the 3' side of the forward primer for the multiplex PCR and a reverse primer wherein a complementary sequence of 10-30 bases on the 3' terminal of the domain selected in the step(a) is joined to the 3' side of the reverse primer, and (c) a PCR reaction is conducted using the primer couple synthesized in the step(b). 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SOLUTION: Each of the primer couples are prepared by the following steps(a) to (c): (a) In an available DNA with a known sequence, a domain having any number of bases between 50 and 2,000 is selected, (b) the primer couple is synthesized, which is composed of a forward primer wherein 10-30 bases on the 5' terminal of the domain selected in the step(a) are joined to the 3' side of the forward primer for the multiplex PCR and a reverse primer wherein a complementary sequence of 10-30 bases on the 3' terminal of the domain selected in the step(a) is joined to the 3' side of the reverse primer, and (c) a PCR reaction is conducted using the primer couple synthesized in the step(b). 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD FOR PREPARING ARTIFICIAL POSITIVE CONTROL DNA GROUP FOR MULTIPLEX PCR |
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