METHOD FOR METHYLATING EXOGENOUS GENE

PROBLEM TO BE SOLVED: To provide a method capable of controlling the copy number of an introduced exogenous gene in creation of a transformed plant and to provide a promoter enabling expression of the exogenous gene in the root in addition to callus. SOLUTION: The inventors found that methylation of...

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Hauptverfasser:	MINOBE YUZO, MANO HIRONORI, TSURUYA YOSHIYO, HIGO HIROMI
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method capable of controlling the copy number of an introduced exogenous gene in creation of a transformed plant and to provide a promoter enabling expression of the exogenous gene in the root in addition to callus. SOLUTION: The inventors found that methylation of the exogenous gene is enhanced according to the copy number by utilizing a plurality of enhancers containing the same base sequence. As a result, in the transformant having many introduction copy numbers, expression level of the exogenous gene is lowered. When the exogenous gene is a selection marker, an individual having low copy number can preferentially be picked out. Further, the present invention provides a promoter capable of inducing gene expression in the root in addition to callus. As a result, root generated from the callus is free from fear blighted and losing a transformant by a selective agent. COPYRIGHT: (C)2007,JPO&INPIT