PRODUCTION OF VASCULAR ENDOTHELIAL CELL GROWTH FACTOR
PROBLEM TO BE SOLVED: To provide a method for producing a wound healing agent that is mitogenic for vascular endothelial cells and consequently is useful in promoting angiogenesis (vasculogenesis) and reendothelialization of inner vascular surfaces as well as a method and means for producing a vascu...
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| creator | MITCHELL RICHARD L TISCHER EDMUND G ABRAHAM JUDITH A FIDDES JOHN C |
| description | PROBLEM TO BE SOLVED: To provide a method for producing a wound healing agent that is mitogenic for vascular endothelial cells and consequently is useful in promoting angiogenesis (vasculogenesis) and reendothelialization of inner vascular surfaces as well as a method and means for producing a vascular endothelial cell growth factor by means of recombinant DNA technology. SOLUTION: Disclosed is an isolated DNA sequence encoding a vascular endothelial cell growth factor of mammals. Disclosed is a method for producing a vascular endothelial cell growth factor, wherein the method comprises the following steps of: (a) culturing a cell transformed by an expression vector comprising a DNA sequence encoding the amino acid sequence of the polypeptide chain of the vascular endothelial cell growth factor under a condition that the peptide can be expressed; (b) collecting the expressed polypeptide; and (c) forming a disulfide bond of the polypeptide chain. COPYRIGHT: (C)2007,JPO&INPIT |
| format | Patent |
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SOLUTION: Disclosed is an isolated DNA sequence encoding a vascular endothelial cell growth factor of mammals. Disclosed is a method for producing a vascular endothelial cell growth factor, wherein the method comprises the following steps of: (a) culturing a cell transformed by an expression vector comprising a DNA sequence encoding the amino acid sequence of the polypeptide chain of the vascular endothelial cell growth factor under a condition that the peptide can be expressed; (b) collecting the expressed polypeptide; and (c) forming a disulfide bond of the polypeptide chain. 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SOLUTION: Disclosed is an isolated DNA sequence encoding a vascular endothelial cell growth factor of mammals. Disclosed is a method for producing a vascular endothelial cell growth factor, wherein the method comprises the following steps of: (a) culturing a cell transformed by an expression vector comprising a DNA sequence encoding the amino acid sequence of the polypeptide chain of the vascular endothelial cell growth factor under a condition that the peptide can be expressed; (b) collecting the expressed polypeptide; and (c) forming a disulfide bond of the polypeptide chain. 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SOLUTION: Disclosed is an isolated DNA sequence encoding a vascular endothelial cell growth factor of mammals. Disclosed is a method for producing a vascular endothelial cell growth factor, wherein the method comprises the following steps of: (a) culturing a cell transformed by an expression vector comprising a DNA sequence encoding the amino acid sequence of the polypeptide chain of the vascular endothelial cell growth factor under a condition that the peptide can be expressed; (b) collecting the expressed polypeptide; and (c) forming a disulfide bond of the polypeptide chain. COPYRIGHT: (C)2007,JPO&INPIT</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | PRODUCTION OF VASCULAR ENDOTHELIAL CELL GROWTH FACTOR |
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