ASSAY FOR CITRUS GREENING DISEASE USING IODINE-STARCH REACTION

PROBLEM TO BE SOLVED: To provide an assay for a citrus greening disease using iodine-starch reaction, a discrimination method soundness, disease and element deficiency based on difference in coloring, a sample treating method, a detecting method for a disease sample based on an iodine addition quantity and an absorbance value, and an assay kit using the methods. SOLUTION: A leaf of a citrus is crushed to be formed into a paste by hot water, its supernatant is thereafter received in a micro plate, an iodine solution is added thereto, and the sample under the disease of the citrus greening disease is colored violet therein, the element deficiency sample is colored pale blue therein, and the sound sample is colored yellow, brown or green therein. The absorbance value is measured in 540 or 655nm of wavelength by an absorbance value measuring instrument such as a micro plate reader. The assay kit is manufactured by a micro centrifugal tube, the micro plate, a pipette, a toothpick, a foamed styrol, and the iodine solution. COPYRIGHT: (C)2004,JPO&NCIPI