ASPERGILLUS ORYZAE npgO GENE

PROBLEM TO BE SOLVED: To provide a phosphopantothenic acid-transferring enzyme gene of Aspergillus oryzae to freely control the production of a secondary metabolite including ferrichrome of the Aspergillus oryzae; to provide a recombinant vector containing the gene, and a transformant containing the...

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Hauptverfasser: ASAI KIYOSHI, KAWATO SHOJI, HATA YOJI, ISHIDA HIROKI, ABE TAKAYOSHI, NAGASAKI HIDEKI, OBATA HIROSHI, GOMI KATSUYA, HOSOYAMA SATORU, AKITA OSAMU, SANO MOTOAKI, OGASAWARA NAOKI, KIN TAISHIN, MACHIDA MASAYUKI, KUHARA SATORU, ABE YASUHISA
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creator ASAI KIYOSHI
KAWATO SHOJI
HATA YOJI
ISHIDA HIROKI
ABE TAKAYOSHI
NAGASAKI HIDEKI
OBATA HIROSHI
GOMI KATSUYA
HOSOYAMA SATORU
AKITA OSAMU
SANO MOTOAKI
OGASAWARA NAOKI
KIN TAISHIN
MACHIDA MASAYUKI
KUHARA SATORU
ABE YASUHISA
description PROBLEM TO BE SOLVED: To provide a phosphopantothenic acid-transferring enzyme gene of Aspergillus oryzae to freely control the production of a secondary metabolite including ferrichrome of the Aspergillus oryzae; to provide a recombinant vector containing the gene, and a transformant containing the recombinant vector; and to provide a non-ribosome type peptide produced by the transformant, and an arbitrary non-ribosome type peptide by using the strain obtained by introducing the recombinant vector to a strain highly expressing an arbitrary NRPS and PKS. SOLUTION: The base sequence of the phosphopantothenic acid-transferring enzyme gene (npgO gene) of Aspergillus oryzae is determined by cloning the gene. The npgO gene is highly expressed with a non-ribosome type peptide synthetase (NRPS) gene (e.g. a peptide synthetase gene asb2 or asb4) to carry out enhanced production of the non-ribosome type peptide (e.g. the ferrichrome of the Aspergillus oryzae) which can not be obtained by singly using the asb2 or the asb4. COPYRIGHT: (C)2004,JPO&NCIPI
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title ASPERGILLUS ORYZAE npgO GENE
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