METHOD FOR CLONING AND PRODUCING AatII RESTRICTION ENDONUCLEASE AND METHYLASE, AND RELATED METHOD FOR OVEREXPRESSING RESTRICTION ENDONUCLEASE
PROBLEM TO BE SOLVED: To provide a new approach to the production of restriction endonucleases. SOLUTION: Provided is a new method for the overexpression of these enzymes, which comprises transforming a host cell with an expression vector containing a DNA encoding the restriction endonuclease and an...
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| creator | XU SHUANG-YONG NWANKWO DONALD O |
| description | PROBLEM TO BE SOLVED: To provide a new approach to the production of restriction endonucleases. SOLUTION: Provided is a new method for the overexpression of these enzymes, which comprises transforming a host cell with an expression vector containing a DNA encoding the restriction endonuclease and an expression vector of a methylase capable of protecting the host cell DNA against the restriction endonuclease. The methylase vector is compatible with the restriction endonuclease expression vector. Preferably, the expression vector is a T7 expression vector, and the host cell is transformed with a third compatible vector which regulates the expression vector. COPYRIGHT: (C)2004,JPO |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD FOR CLONING AND PRODUCING AatII RESTRICTION ENDONUCLEASE AND METHYLASE, AND RELATED METHOD FOR OVEREXPRESSING RESTRICTION ENDONUCLEASE |
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