METHOD FOR DETECTING TARGET NUCLEIC ACID
PROBLEM TO BE SOLVED: To provide a method excellent in operability and capable of rapidly and simply detecting the existence of a target nucleic acid in a sample, and to provide a kit for detecting the target nucleic acid. SOLUTION: This method for detecting the target nucleic acid comprises using a...
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| creator | TANAKA YASUYUKI ASAI RIYOUKO OKADA KEISAKU |
| description | PROBLEM TO BE SOLVED: To provide a method excellent in operability and capable of rapidly and simply detecting the existence of a target nucleic acid in a sample, and to provide a kit for detecting the target nucleic acid. SOLUTION: This method for detecting the target nucleic acid comprises using a capture probe having bondability to the target nucleic acid and a competitive probe having no bondability to the target nucleic acid but having bondability to the capture probe, wherein the method comprises a step for mixing the competitive probe having a ligand with a sample, a step for introducing the obtained mixture on a water-absorbing substrate fixed with the capture probe, a step for introducing a signal reporter on the water-absorbing substrate, a step for developing the mixture and the signal reporter on the water-absorbing substrate, and a step for detecting a signal from the signal reporter. The method determines that, if the lower signal than that of a control sample is detected, the target nucleic acid is contained in the sample. The kit for detecting the target nucleic acid is provided. COPYRIGHT: (C)2003,JPO |
| format | Patent |
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SOLUTION: This method for detecting the target nucleic acid comprises using a capture probe having bondability to the target nucleic acid and a competitive probe having no bondability to the target nucleic acid but having bondability to the capture probe, wherein the method comprises a step for mixing the competitive probe having a ligand with a sample, a step for introducing the obtained mixture on a water-absorbing substrate fixed with the capture probe, a step for introducing a signal reporter on the water-absorbing substrate, a step for developing the mixture and the signal reporter on the water-absorbing substrate, and a step for detecting a signal from the signal reporter. The method determines that, if the lower signal than that of a control sample is detected, the target nucleic acid is contained in the sample. The kit for detecting the target nucleic acid is provided. 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SOLUTION: This method for detecting the target nucleic acid comprises using a capture probe having bondability to the target nucleic acid and a competitive probe having no bondability to the target nucleic acid but having bondability to the capture probe, wherein the method comprises a step for mixing the competitive probe having a ligand with a sample, a step for introducing the obtained mixture on a water-absorbing substrate fixed with the capture probe, a step for introducing a signal reporter on the water-absorbing substrate, a step for developing the mixture and the signal reporter on the water-absorbing substrate, and a step for detecting a signal from the signal reporter. The method determines that, if the lower signal than that of a control sample is detected, the target nucleic acid is contained in the sample. The kit for detecting the target nucleic acid is provided. 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SOLUTION: This method for detecting the target nucleic acid comprises using a capture probe having bondability to the target nucleic acid and a competitive probe having no bondability to the target nucleic acid but having bondability to the capture probe, wherein the method comprises a step for mixing the competitive probe having a ligand with a sample, a step for introducing the obtained mixture on a water-absorbing substrate fixed with the capture probe, a step for introducing a signal reporter on the water-absorbing substrate, a step for developing the mixture and the signal reporter on the water-absorbing substrate, and a step for detecting a signal from the signal reporter. The method determines that, if the lower signal than that of a control sample is detected, the target nucleic acid is contained in the sample. The kit for detecting the target nucleic acid is provided. COPYRIGHT: (C)2003,JPO</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD FOR DETECTING TARGET NUCLEIC ACID |
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