ISOLATION OF RHODOBACTER SPHAEROIDES RV DOUBLE VARIANT DEFICIENT IN BOTH PHA PRODUCTION AND UPTAKE TYPE HYDROGENASE, HAVING INCREASED HYDROGEN PHOTO- PRODUCTIVITY

PROBLEM TO BE SOLVED: To obtain the subject new double variant useful for producing hydrogen, comprising a variant of a specific Rhodobacter sphaeroides RV incapable of accumulating a polyhydroxyalkanoic acid ester and incapable of expressing hydrogenase uptake activity. SOLUTION: This variant of Rh...

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Hauptverfasser:	SCOLLA GIUSEPPE, FRANCESCO RODRIGUEZ, TOSI CLAUDIO, PEDRONI PAOLA, GINO DERA PENNA, ELISABETTA FRANCHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DISPOSAL OF SOLID WASTE ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECLAMATION OF CONTAMINED SOIL SOIL SPIRITS TRANSPORTING VINEGAR WINE
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Zusammenfassung:	PROBLEM TO BE SOLVED: To obtain the subject new double variant useful for producing hydrogen, comprising a variant of a specific Rhodobacter sphaeroides RV incapable of accumulating a polyhydroxyalkanoic acid ester and incapable of expressing hydrogenase uptake activity. SOLUTION: This variant of Rhodobacter sphaeroides RV is incapable of accumulating a polyhydroxyalkanoic acid ester and incapable of expressing hydrogenase uptake activity, deposited with the association number CBS 101324 has an improved ability of producing hydrogen and is useful for producing hydrogen. The variant is obtained by inactivating a gene encoding a large subunit of uptake type hydrogenase enzyme of Rhodobacter sphaeroides and a gene encoding a synthase enzyme by two continuous mutageneses by random mutagenesis by using transposon or specific transposon by interposon.