PRODUCTION OF S-HYDROXYNITRILELYASE

PRODUCTION OF S-HYDROXYNITRILELYASE

PROBLEM TO BE SOLVED: To obtain an S-hydroxynitrilelyase useful synthesizing an optically active S-cyanohydrin from a carbonyl compound with hydrocyanic acid in a good efficiency, high purity and high yield by culturing a specific yeast in a medium. SOLUTION: This method for producing an S-hydroxyni...

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1. Verfasser: SENBA TAKASHI
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BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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creator SENBA TAKASHI
description PROBLEM TO BE SOLVED: To obtain an S-hydroxynitrilelyase useful synthesizing an optically active S-cyanohydrin from a carbonyl compound with hydrocyanic acid in a good efficiency, high purity and high yield by culturing a specific yeast in a medium. SOLUTION: This method for producing an S-hydroxynitrilelyase is to culture an yeast belonging to the genus Pichia and transformed by a recombinant DNA obtained by incorporating a gene encoding the S-hydroxynitrilelyase (EC-4. 1. 2. 37) originated from a cassava, into an yeast chromosome-incorporating type expression vector. Further, it is preferable that the yeast chromosome- incorporating type expression vector consists of a DNA consisting of a base sequence of a AOX1 (alcohol oxidase) promoter originating from the yeast belonging to the genus Pichia, or a base sequence in which one or several bases are lost, substituted or added in the above base sequence, and containing the DNA maintaining the promoter activity.
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SOLUTION: This method for producing an S-hydroxynitrilelyase is to culture an yeast belonging to the genus Pichia and transformed by a recombinant DNA obtained by incorporating a gene encoding the S-hydroxynitrilelyase (EC-4. 1. 2. 37) originated from a cassava, into an yeast chromosome-incorporating type expression vector. 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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title PRODUCTION OF S-HYDROXYNITRILELYASE
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