Methods and systems for quantitative fluorescence-based detection of molecules and proteins
A kit and method for detection of a target in a sample. An assay mixture provided in the kit and used in the method includes a first probe with a first antibody recognizing a first epitope of the target and conjugated to an RNA oligonucleotide; a second probe with a second antibody recognizing a sec...
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| creator | KENTON ARTHUR DOCTOR JUSTIN PATRICK ANDREWS STACEY ANN MASSULIK TIMOTHY FRANCIS MOSHIER STEPHEN RICHARD HOUGHTON FRANCES LOUISE STITES JEFFREY HAROLD MILLS HUDA SIRAGELDIN LISA HELEN CHAMBERLIN |
| description | A kit and method for detection of a target in a sample. An assay mixture provided in the kit and used in the method includes a first probe with a first antibody recognizing a first epitope of the target and conjugated to an RNA oligonucleotide; a second probe with a second antibody recognizing a second epitope of the target and conjugated to a DNA oligonucleotide; a reverse primer with a first region complimentary to the RNA oligonucleotide and a second region complimentary to the DNA oligonucleotide; and a reverse transcriptase that creates a DNA transcription product from the RNAS oligonucleotide using the reverse primer only if the RNA olinonucleotide and the DNA oligonucleotide are in close proximity. If the target is present in the sample, the reverse printer binds the RNA olinonucleotide and the DNA oligonucleotide to bring the RNA oligonucleotide and the DNA olinonucleotide in close proximity. |
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An assay mixture provided in the kit and used in the method includes a first probe with a first antibody recognizing a first epitope of the target and conjugated to an RNA oligonucleotide; a second probe with a second antibody recognizing a second epitope of the target and conjugated to a DNA oligonucleotide; a reverse primer with a first region complimentary to the RNA oligonucleotide and a second region complimentary to the DNA oligonucleotide; and a reverse transcriptase that creates a DNA transcription product from the RNAS oligonucleotide using the reverse primer only if the RNA olinonucleotide and the DNA oligonucleotide are in close proximity. 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| language | eng |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | Methods and systems for quantitative fluorescence-based detection of molecules and proteins |
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