PROCESS FOR CLONING FRAGMENT ENCODING THE SYNTHESIS OF TETRACYCLINE AND CHLOROTETRACYCLINE AND FOR EXPRESSING CLONED FRAGMENTS

The present invention relates to the cloning, isolation and DNA (isolated fragment from Streptomyces aureofaciens) which directs the synthesis of tetracycline and chlortetracycline, in a heterologous host such as Streptomyces lividans. The claimed plasmids are isolated by screening a cosmid library,...

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Hauptverfasser: RYAN,MICHALE J.,US, LOTVIN,JASON A.,US, STRATHY,NANCY,US
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creator RYAN,MICHALE J.,US
LOTVIN,JASON A.,US
STRATHY,NANCY,US
description The present invention relates to the cloning, isolation and DNA (isolated fragment from Streptomyces aureofaciens) which directs the synthesis of tetracycline and chlortetracycline, in a heterologous host such as Streptomyces lividans. The claimed plasmids are isolated by screening a cosmid library, constructed in Escherichia coli, for the expression of tetracycline resistance in S. lividans.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title PROCESS FOR CLONING FRAGMENT ENCODING THE SYNTHESIS OF TETRACYCLINE AND CHLOROTETRACYCLINE AND FOR EXPRESSING CLONED FRAGMENTS
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