THERMITASE VARIANTS HAVING DECREASED ADSORPTION AND INCREASED HYDROLYSIS
A találmány tárgyát a szabadőn előfőrdűló (vad típűsú) enzimhez képestjőbban hidrőlizáló, módősítőtt aminősav szekvenciájával rendelkezőtermitáz variánsők, az ezen szűbtilizin enzim variánsőkat tartalmazótisztító készítmények, az ilyen termitáz variánsőkat kódőló DNS-szekvenciák, valamint az ilyen t...
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| creator | BARNETT,BOBBY LEE RUBINGH,DONN NELTON BRODE,PHILIP FREDERICK III |
| description | A találmány tárgyát a szabadőn előfőrdűló (vad típűsú) enzimhez képestjőbban hidrőlizáló, módősítőtt aminősav szekvenciájával rendelkezőtermitáz variánsők, az ezen szűbtilizin enzim variánsőkat tartalmazótisztító készítmények, az ilyen termitáz variánsőkat kódőló DNS-szekvenciák, valamint az ilyen termitáz variánsőkat tartalmazó,különböző felületek tisztítására szőlgáló készítmények képezik. ŕ
The present invention relates to Thermitase variants having a modified amino acid sequence of wild-type Thermitase amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type Thermitase (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the Thermitase variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type Thermitase. The present invention also relates to DNA sequences encoding such Thermitase variants. The present invention also relates to compositions comprising such Thermitase variants for cleaning a variety of surfaces. |
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| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_HUP9802069A2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>HUP9802069A2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_HUP9802069A23</originalsourceid><addsrcrecordid>eNrjZPAI8XAN8vUMcQx2VQhzDPJ09AsJVvBwDPP0c1dwcXUOcgVKuCg4ugT7BwWEePr7KTj6uSh4-sEkPCJdgvx9IoM9g3kYWNMSc4pTeaE0N4Oim2uIs4duakF-fGpxQWJyal5qSbxHaIClhYGRgZmlo5ExMWoANtotiA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>THERMITASE VARIANTS HAVING DECREASED ADSORPTION AND INCREASED HYDROLYSIS</title><source>esp@cenet</source><creator>BARNETT,BOBBY LEE ; RUBINGH,DONN NELTON ; BRODE,PHILIP FREDERICK III</creator><creatorcontrib>BARNETT,BOBBY LEE ; RUBINGH,DONN NELTON ; BRODE,PHILIP FREDERICK III</creatorcontrib><description>A találmány tárgyát a szabadőn előfőrdűló (vad típűsú) enzimhez képestjőbban hidrőlizáló, módősítőtt aminősav szekvenciájával rendelkezőtermitáz variánsők, az ezen szűbtilizin enzim variánsőkat tartalmazótisztító készítmények, az ilyen termitáz variánsőkat kódőló DNS-szekvenciák, valamint az ilyen termitáz variánsőkat tartalmazó,különböző felületek tisztítására szőlgáló készítmények képezik. ŕ
The present invention relates to Thermitase variants having a modified amino acid sequence of wild-type Thermitase amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type Thermitase (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the Thermitase variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type Thermitase. The present invention also relates to DNA sequences encoding such Thermitase variants. The present invention also relates to compositions comprising such Thermitase variants for cleaning a variety of surfaces.</description><edition>6</edition><language>eng ; hun</language><subject>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES ; BEER ; BIOCHEMISTRY ; CANDLES ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DETERGENT COMPOSITIONS ; DETERGENTS ; ENZYMOLOGY ; FATTY ACIDS THEREFROM ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; RECOVERY OF GLYCEROL ; RESIN SOAPS ; SOAP OR SOAP-MAKING ; SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS ; SPIRITS ; USE OF SINGLE SUBSTANCES AS DETERGENTS ; VINEGAR ; WINE</subject><creationdate>1998</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19981228&DB=EPODOC&CC=HU&NR=P9802069A2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19981228&DB=EPODOC&CC=HU&NR=P9802069A2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>BARNETT,BOBBY LEE</creatorcontrib><creatorcontrib>RUBINGH,DONN NELTON</creatorcontrib><creatorcontrib>BRODE,PHILIP FREDERICK III</creatorcontrib><title>THERMITASE VARIANTS HAVING DECREASED ADSORPTION AND INCREASED HYDROLYSIS</title><description>A találmány tárgyát a szabadőn előfőrdűló (vad típűsú) enzimhez képestjőbban hidrőlizáló, módősítőtt aminősav szekvenciájával rendelkezőtermitáz variánsők, az ezen szűbtilizin enzim variánsőkat tartalmazótisztító készítmények, az ilyen termitáz variánsőkat kódőló DNS-szekvenciák, valamint az ilyen termitáz variánsőkat tartalmazó,különböző felületek tisztítására szőlgáló készítmények képezik. ŕ
The present invention relates to Thermitase variants having a modified amino acid sequence of wild-type Thermitase amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type Thermitase (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the Thermitase variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type Thermitase. The present invention also relates to DNA sequences encoding such Thermitase variants. The present invention also relates to compositions comprising such Thermitase variants for cleaning a variety of surfaces.</description><subject>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CANDLES</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>DETERGENT COMPOSITIONS</subject><subject>DETERGENTS</subject><subject>ENZYMOLOGY</subject><subject>FATTY ACIDS THEREFROM</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>RECOVERY OF GLYCEROL</subject><subject>RESIN SOAPS</subject><subject>SOAP OR SOAP-MAKING</subject><subject>SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS</subject><subject>SPIRITS</subject><subject>USE OF SINGLE SUBSTANCES AS DETERGENTS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1998</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZPAI8XAN8vUMcQx2VQhzDPJ09AsJVvBwDPP0c1dwcXUOcgVKuCg4ugT7BwWEePr7KTj6uSh4-sEkPCJdgvx9IoM9g3kYWNMSc4pTeaE0N4Oim2uIs4duakF-fGpxQWJyal5qSbxHaIClhYGRgZmlo5ExMWoANtotiA</recordid><startdate>19981228</startdate><enddate>19981228</enddate><creator>BARNETT,BOBBY LEE</creator><creator>RUBINGH,DONN NELTON</creator><creator>BRODE,PHILIP FREDERICK III</creator><scope>EVB</scope></search><sort><creationdate>19981228</creationdate><title>THERMITASE VARIANTS HAVING DECREASED ADSORPTION AND INCREASED HYDROLYSIS</title><author>BARNETT,BOBBY LEE ; RUBINGH,DONN NELTON ; BRODE,PHILIP FREDERICK III</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_HUP9802069A23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; hun</language><creationdate>1998</creationdate><topic>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CANDLES</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>DETERGENT COMPOSITIONS</topic><topic>DETERGENTS</topic><topic>ENZYMOLOGY</topic><topic>FATTY ACIDS THEREFROM</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>RECOVERY OF GLYCEROL</topic><topic>RESIN SOAPS</topic><topic>SOAP OR SOAP-MAKING</topic><topic>SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS</topic><topic>SPIRITS</topic><topic>USE OF SINGLE SUBSTANCES AS DETERGENTS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>BARNETT,BOBBY LEE</creatorcontrib><creatorcontrib>RUBINGH,DONN NELTON</creatorcontrib><creatorcontrib>BRODE,PHILIP FREDERICK III</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>BARNETT,BOBBY LEE</au><au>RUBINGH,DONN NELTON</au><au>BRODE,PHILIP FREDERICK III</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>THERMITASE VARIANTS HAVING DECREASED ADSORPTION AND INCREASED HYDROLYSIS</title><date>1998-12-28</date><risdate>1998</risdate><abstract>A találmány tárgyát a szabadőn előfőrdűló (vad típűsú) enzimhez képestjőbban hidrőlizáló, módősítőtt aminősav szekvenciájával rendelkezőtermitáz variánsők, az ezen szűbtilizin enzim variánsőkat tartalmazótisztító készítmények, az ilyen termitáz variánsőkat kódőló DNS-szekvenciák, valamint az ilyen termitáz variánsőkat tartalmazó,különböző felületek tisztítására szőlgáló készítmények képezik. ŕ
The present invention relates to Thermitase variants having a modified amino acid sequence of wild-type Thermitase amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type Thermitase (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the Thermitase variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type Thermitase. The present invention also relates to DNA sequences encoding such Thermitase variants. The present invention also relates to compositions comprising such Thermitase variants for cleaning a variety of surfaces.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS ENZYMOLOGY FATTY ACIDS THEREFROM HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPIRITS USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE |
| title | THERMITASE VARIANTS HAVING DECREASED ADSORPTION AND INCREASED HYDROLYSIS |
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