An improved mutagenesis method using polyethylene glycol mediated introduction of mutagenic nucleobases into plant protoplasts

Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the compleme...

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Hauptverfasser:	Bundock, Paul, de Both, Michiel Theodoor Jan, Lhuissier, Franck Georges Paul
Format:	Patent
Sprache:	eng ; hun
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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