Method and system for sampling of airborne microbial activity
An airborne microbial detection system comprises a housing comprising an air sampler 105 configured to collect and process ambient air to create a liquid sample, the air sampler comprising an air inlet (305, fig 3) and a liquid outlet (330, fig 3). The housing also comprises a removable cassette 130...
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| creator | Stephen Daniels Michael Gordon Robert Merriman Ruairi Monaghan |
| description | An airborne microbial detection system comprises a housing comprising an air sampler 105 configured to collect and process ambient air to create a liquid sample, the air sampler comprising an air inlet (305, fig 3) and a liquid outlet (330, fig 3). The housing also comprises a removable cassette 130 that has a plurality of vials (210, fig 2a) for receiving a volume of liquid. A first vial is configured for receiving the liquid sample from the air sampler. At least one other vial of the plurality of vials comprises a reagent for a microbial assay and the system is configured to effect an introduction of at least a portion of the liquid sample into the at least one other vial to create a sample under test. A lysing station 120 is also provided so that the sample under test can be lysed, and a monitoring station is provided for determining optical changes in the sample under test and to provide an output indicative of monitored optical changes. The system is configured to effect a movement of the cassette within the housing for selective presentation of the cassette to each of the air sampler, lysing station and monitoring station 150. Preferably, the assay is a loop mediated isothermal amplification (LAMP) assay. |
| format | Patent |
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The housing also comprises a removable cassette 130 that has a plurality of vials (210, fig 2a) for receiving a volume of liquid. A first vial is configured for receiving the liquid sample from the air sampler. At least one other vial of the plurality of vials comprises a reagent for a microbial assay and the system is configured to effect an introduction of at least a portion of the liquid sample into the at least one other vial to create a sample under test. A lysing station 120 is also provided so that the sample under test can be lysed, and a monitoring station is provided for determining optical changes in the sample under test and to provide an output indicative of monitored optical changes. The system is configured to effect a movement of the cassette within the housing for selective presentation of the cassette to each of the air sampler, lysing station and monitoring station 150. 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The housing also comprises a removable cassette 130 that has a plurality of vials (210, fig 2a) for receiving a volume of liquid. A first vial is configured for receiving the liquid sample from the air sampler. At least one other vial of the plurality of vials comprises a reagent for a microbial assay and the system is configured to effect an introduction of at least a portion of the liquid sample into the at least one other vial to create a sample under test. A lysing station 120 is also provided so that the sample under test can be lysed, and a monitoring station is provided for determining optical changes in the sample under test and to provide an output indicative of monitored optical changes. The system is configured to effect a movement of the cassette within the housing for selective presentation of the cassette to each of the air sampler, lysing station and monitoring station 150. Preferably, the assay is a loop mediated isothermal amplification (LAMP) assay.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | Method and system for sampling of airborne microbial activity |
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