Expression of heterologous enzymes in yeast for flavoured alcoholic beverage production

A process for preparing flavoured and fermented alcoholic beverages comprising the use of recombinant yeast host cells expressing one or more heterologous enzymes for making a flavour compound and a native ethanol production pathway. The flavour compound may be lactic acid (using lactate dehydrogena...

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Hauptverfasser: Bailey Morgan Carignan, Hannah Lena Green, Brooks Henningsen, Aaron Argyros, Emily Agnes Stonehouse, Christopher J Freeman, Charles F Rice, Nicholas Memmer, Jared Cameron Muysson
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creator Bailey Morgan Carignan
Hannah Lena Green
Brooks Henningsen
Aaron Argyros
Emily Agnes Stonehouse
Christopher J Freeman
Charles F Rice
Nicholas Memmer
Jared Cameron Muysson
description A process for preparing flavoured and fermented alcoholic beverages comprising the use of recombinant yeast host cells expressing one or more heterologous enzymes for making a flavour compound and a native ethanol production pathway. The flavour compound may be lactic acid (using lactate dehydrogenase or mutated malate dehydrogenase), vanillin (using feruloyl-CoA-synthase and enoyl-CoA-hydratase), isoamyl acetate (using alcohol acetyl transferase) or 4-(4-hydroxyphenyl)​-2-butanone (using phenylalanine-ammonium lyase, cinnimate-4-hydroxylase, coumarate-CoA ligase and benzalacetone synthase). The recombinant yeast host cells can be used in a subsequent production process to make flavoured alcoholic beverage products, such as beers.
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subjects BEER
BIOCHEMISTRY
BREWING OF BEER
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
OTHER ALCOHOLIC BEVERAGES
PREPARATION THEREOF
SPIRITS
VINEGAR
WINE
title Expression of heterologous enzymes in yeast for flavoured alcoholic beverage production
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