Stable cell lines for inducible production of rAAV virions
Production of recombinant adeno-associated virus (AAV) via the expression of a nucleic acid encoding Rep and Cap proteins and a second nucleic acid encoding adenoviral helper proteins, such as E2A and E4. Either nucleic acid is conditionally expressed. Exemplified is recombinant AAV production in HE...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Patent |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | Kenneth Prentice Sandhya Pande |
| description | Production of recombinant adeno-associated virus (AAV) via the expression of a nucleic acid encoding Rep and Cap proteins and a second nucleic acid encoding adenoviral helper proteins, such as E2A and E4. Either nucleic acid is conditionally expressed. Exemplified is recombinant AAV production in HEK 293 cells via an inducible Cre/LoxP system wherein the Rep gene comprises an intron flanked by LoxP sites. Rep is conditionally expressed due to Rep toxicity. A second plasmid comprises E2A and E4 genes and an inducible Cre recombinase. Cre expression is induced via tamoxifen which excises the disruptive intron to induce Rep protein expression. There is also a plasmid comprises a transgene for packaging into the AAV, for example for gene therapy. The Rep intron may comprise a marker, such as GFP. The cells may also express VA genes and auxotrophic selection markers, such as DHFR. The DHFR sequence may be split across two sequences and functional DHFR formed via a leucine zipper for selection. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_GB2599212A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>GB2599212A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_GB2599212A3</originalsourceid><addsrcrecordid>eNrjZLAKLklMyklVSE7NyVHIycxLLVZIyy9SyMxLKU3OBEkUFOUDmSWZ-XkK-WkKRY6OYQplmUVAbjEPA2taYk5xKi-U5maQd3MNcfbQTS3Ij08tLkhMTs1LLYl3dzIytbQ0MjRyNCasAgCQqC3F</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Stable cell lines for inducible production of rAAV virions</title><source>esp@cenet</source><creator>Kenneth Prentice ; Sandhya Pande</creator><creatorcontrib>Kenneth Prentice ; Sandhya Pande</creatorcontrib><description>Production of recombinant adeno-associated virus (AAV) via the expression of a nucleic acid encoding Rep and Cap proteins and a second nucleic acid encoding adenoviral helper proteins, such as E2A and E4. Either nucleic acid is conditionally expressed. Exemplified is recombinant AAV production in HEK 293 cells via an inducible Cre/LoxP system wherein the Rep gene comprises an intron flanked by LoxP sites. Rep is conditionally expressed due to Rep toxicity. A second plasmid comprises E2A and E4 genes and an inducible Cre recombinase. Cre expression is induced via tamoxifen which excises the disruptive intron to induce Rep protein expression. There is also a plasmid comprises a transgene for packaging into the AAV, for example for gene therapy. The Rep intron may comprise a marker, such as GFP. The cells may also express VA genes and auxotrophic selection markers, such as DHFR. The DHFR sequence may be split across two sequences and functional DHFR formed via a leucine zipper for selection.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220330&DB=EPODOC&CC=GB&NR=2599212A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220330&DB=EPODOC&CC=GB&NR=2599212A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Kenneth Prentice</creatorcontrib><creatorcontrib>Sandhya Pande</creatorcontrib><title>Stable cell lines for inducible production of rAAV virions</title><description>Production of recombinant adeno-associated virus (AAV) via the expression of a nucleic acid encoding Rep and Cap proteins and a second nucleic acid encoding adenoviral helper proteins, such as E2A and E4. Either nucleic acid is conditionally expressed. Exemplified is recombinant AAV production in HEK 293 cells via an inducible Cre/LoxP system wherein the Rep gene comprises an intron flanked by LoxP sites. Rep is conditionally expressed due to Rep toxicity. A second plasmid comprises E2A and E4 genes and an inducible Cre recombinase. Cre expression is induced via tamoxifen which excises the disruptive intron to induce Rep protein expression. There is also a plasmid comprises a transgene for packaging into the AAV, for example for gene therapy. The Rep intron may comprise a marker, such as GFP. The cells may also express VA genes and auxotrophic selection markers, such as DHFR. The DHFR sequence may be split across two sequences and functional DHFR formed via a leucine zipper for selection.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLAKLklMyklVSE7NyVHIycxLLVZIyy9SyMxLKU3OBEkUFOUDmSWZ-XkK-WkKRY6OYQplmUVAbjEPA2taYk5xKi-U5maQd3MNcfbQTS3Ij08tLkhMTs1LLYl3dzIytbQ0MjRyNCasAgCQqC3F</recordid><startdate>20220330</startdate><enddate>20220330</enddate><creator>Kenneth Prentice</creator><creator>Sandhya Pande</creator><scope>EVB</scope></search><sort><creationdate>20220330</creationdate><title>Stable cell lines for inducible production of rAAV virions</title><author>Kenneth Prentice ; Sandhya Pande</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_GB2599212A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Kenneth Prentice</creatorcontrib><creatorcontrib>Sandhya Pande</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Kenneth Prentice</au><au>Sandhya Pande</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Stable cell lines for inducible production of rAAV virions</title><date>2022-03-30</date><risdate>2022</risdate><abstract>Production of recombinant adeno-associated virus (AAV) via the expression of a nucleic acid encoding Rep and Cap proteins and a second nucleic acid encoding adenoviral helper proteins, such as E2A and E4. Either nucleic acid is conditionally expressed. Exemplified is recombinant AAV production in HEK 293 cells via an inducible Cre/LoxP system wherein the Rep gene comprises an intron flanked by LoxP sites. Rep is conditionally expressed due to Rep toxicity. A second plasmid comprises E2A and E4 genes and an inducible Cre recombinase. Cre expression is induced via tamoxifen which excises the disruptive intron to induce Rep protein expression. There is also a plasmid comprises a transgene for packaging into the AAV, for example for gene therapy. The Rep intron may comprise a marker, such as GFP. The cells may also express VA genes and auxotrophic selection markers, such as DHFR. The DHFR sequence may be split across two sequences and functional DHFR formed via a leucine zipper for selection.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_epo_espacenet_GB2599212A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Stable cell lines for inducible production of rAAV virions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T15%3A14%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=Kenneth%20Prentice&rft.date=2022-03-30&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EGB2599212A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |