Purification method
Method of isolating nucleic acid from a sample, comprising: (i) contacting a sample comprising nucleic acid with an alcohol and a salt; (ii) contacting a filter with the composition comprising nucleic acid obtained following step (i) to provide a filter having nucleic acid bonded thereto; and (iv) e...
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| creator | Georgios Patsos |
| description | Method of isolating nucleic acid from a sample, comprising: (i) contacting a sample comprising nucleic acid with an alcohol and a salt; (ii) contacting a filter with the composition comprising nucleic acid obtained following step (i) to provide a filter having nucleic acid bonded thereto; and (iv) eluting at least a portion of the nucleic acid from the filter, wherein elution comprises incubating the filter in an elution buffer at a temperature of from 70 to 90 ºC. Optionally, between steps (ii) and (iv), step (iii) treating the filter having the nucleic acid bonded to it with a wash solution comprising alcohol. Optionally, the elution temperature is 80 ºC. Optionally, said filter can have at least a first porous region and a second porous region; the first porous region being arranged to be contacted in use by the sample before the second porous region, wherein the first porous region has a nominal pore size greater than the second porous region. |
| format | Patent |
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Optionally, between steps (ii) and (iv), step (iii) treating the filter having the nucleic acid bonded to it with a wash solution comprising alcohol. Optionally, the elution temperature is 80 ºC. Optionally, said filter can have at least a first porous region and a second porous region; the first porous region being arranged to be contacted in use by the sample before the second porous region, wherein the first porous region has a nominal pore size greater than the second porous region.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201209&DB=EPODOC&CC=GB&NR=2584562A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201209&DB=EPODOC&CC=GB&NR=2584562A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Georgios Patsos</creatorcontrib><title>Purification method</title><description>Method of isolating nucleic acid from a sample, comprising: (i) contacting a sample comprising nucleic acid with an alcohol and a salt; (ii) contacting a filter with the composition comprising nucleic acid obtained following step (i) to provide a filter having nucleic acid bonded thereto; and (iv) eluting at least a portion of the nucleic acid from the filter, wherein elution comprises incubating the filter in an elution buffer at a temperature of from 70 to 90 ºC. Optionally, between steps (ii) and (iv), step (iii) treating the filter having the nucleic acid bonded to it with a wash solution comprising alcohol. Optionally, the elution temperature is 80 ºC. Optionally, said filter can have at least a first porous region and a second porous region; the first porous region being arranged to be contacted in use by the sample before the second porous region, wherein the first porous region has a nominal pore size greater than the second porous region.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZBAOKC3KTMtMTizJzM9TyE0tychP4WFgTUvMKU7lhdLcDPJuriHOHrqpBfnxqcUFicmpeakl8e5ORqYWJqZmRo7GhFUAALhvH7Q</recordid><startdate>20201209</startdate><enddate>20201209</enddate><creator>Georgios Patsos</creator><scope>EVB</scope></search><sort><creationdate>20201209</creationdate><title>Purification method</title><author>Georgios Patsos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_GB2584562A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Georgios Patsos</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Georgios Patsos</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Purification method</title><date>2020-12-09</date><risdate>2020</risdate><abstract>Method of isolating nucleic acid from a sample, comprising: (i) contacting a sample comprising nucleic acid with an alcohol and a salt; (ii) contacting a filter with the composition comprising nucleic acid obtained following step (i) to provide a filter having nucleic acid bonded thereto; and (iv) eluting at least a portion of the nucleic acid from the filter, wherein elution comprises incubating the filter in an elution buffer at a temperature of from 70 to 90 ºC. Optionally, between steps (ii) and (iv), step (iii) treating the filter having the nucleic acid bonded to it with a wash solution comprising alcohol. Optionally, the elution temperature is 80 ºC. Optionally, said filter can have at least a first porous region and a second porous region; the first porous region being arranged to be contacted in use by the sample before the second porous region, wherein the first porous region has a nominal pore size greater than the second porous region.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Purification method |
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