Droplet-based assay system

A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsion...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	BILLY WAYNE COLSTON JR, FRED PAUL MILANOVICH, DOUGLAS N MODLIN, VINCENT RIOT, ANTHONY JOSEPH MARKAREWICZ, BENJAMIN JOSEPH HINDSON, SAMUEL BURD, DONALD ARTHUR MASQUELIER, KEVIN DEAN NESS
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.