Cell culture support

A cell culture support for culturing mesenchymal stem cells comprises an upper surface comprising a plurality of wells, wherein the surface has a root mean square roughness Rq of 100-280nm and a linear density of 1.6-3.0 per 1 Î¼m length. The wells preferably have an opening size of 70-550 Î¼m. The...

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Hauptverfasser:	SHUNSUKE TAKEI, YASUHIKO TABATA, TAKAFUMI IMAIZUMI, FUMIHIKO KITAGAWA, ITSUKI YAMAMOTO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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creator	SHUNSUKE TAKEI YASUHIKO TABATA TAKAFUMI IMAIZUMI FUMIHIKO KITAGAWA ITSUKI YAMAMOTO
description	A cell culture support for culturing mesenchymal stem cells comprises an upper surface comprising a plurality of wells, wherein the surface has a root mean square roughness Rq of 100-280nm and a linear density of 1.6-3.0 per 1 Î¼m length. The wells preferably have an opening size of 70-550 Î¼m. The support preferably comprises sintered ceramics, and in preferably sintered zirconia with an average particle size of 0.6-0.9 Î¼m. A method of culturing cells comprises disposing the cell culture support in a container, supplying a first culture medium to the container to allow it to permeate the wells of the support, supplying a second culture medium (which may be the same as the first) comprising undifferentiated mesenchymal stem cells dropwise to the upper surface of the support, supplying more of the first culture medium to the container to immerse the support and to allow aggregation of the mesenchymal stem cells, discharging the medium and supplying a third culture medium for inducing differentiation of the aggregated mesenchymal stem cells into hyaline chondrocytes, adipocytes or osteoblasts.
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The wells preferably have an opening size of 70-550 Î¼m. The support preferably comprises sintered ceramics, and in preferably sintered zirconia with an average particle size of 0.6-0.9 Î¼m. A method of culturing cells comprises disposing the cell culture support in a container, supplying a first culture medium to the container to allow it to permeate the wells of the support, supplying a second culture medium (which may be the same as the first) comprising undifferentiated mesenchymal stem cells dropwise to the upper surface of the support, supplying more of the first culture medium to the container to immerse the support and to allow aggregation of the mesenchymal stem cells, discharging the medium and supplying a third culture medium for inducing differentiation of the aggregated mesenchymal stem cells into hyaline chondrocytes, adipocytes or osteoblasts.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120208&DB=EPODOC&CC=GB&NR=2482612A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120208&DB=EPODOC&CC=GB&NR=2482612A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SHUNSUKE TAKEI</creatorcontrib><creatorcontrib>YASUHIKO TABATA</creatorcontrib><creatorcontrib>TAKAFUMI IMAIZUMI</creatorcontrib><creatorcontrib>FUMIHIKO KITAGAWA</creatorcontrib><creatorcontrib>ITSUKI YAMAMOTO</creatorcontrib><title>Cell culture support</title><description>A cell culture support for culturing mesenchymal stem cells comprises an upper surface comprising a plurality of wells, wherein the surface has a root mean square roughness Rq of 100-280nm and a linear density of 1.6-3.0 per 1 Î¼m length. The wells preferably have an opening size of 70-550 Î¼m. The support preferably comprises sintered ceramics, and in preferably sintered zirconia with an average particle size of 0.6-0.9 Î¼m. 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subjects	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
title	Cell culture support
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