Cell suspension separation method
The invention relates to a method for the separation of suspended cells comprising the steps of discharging a volume of a cell suspension from a bioreactor, applying the volume to a separation device, separating the cells from the suspending liquid, quenching the separated cells in the separation de...
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| creator | MARTIN VOLMER TOBIAS THUELE STEFAN NORTHOFF THOMAS NOLL HEINO BUENTEMEYER |
| description | The invention relates to a method for the separation of suspended cells comprising the steps of discharging a volume of a cell suspension from a bioreactor, applying the volume to a separation device, separating the cells from the suspending liquid, quenching the separated cells in the separation device at a temperature in the range of 100 to -60°C and discharging said quenched and separated cells from the separation device, wherein the separation and quenching steps are conducted in a common step. The quenching ensures most of the metabolic processes within the cells are stopped. The separation device may be a filtration means and the separation and quenching steps may be achieved with a chilled wash solution. The separated cells, which may be animal or insect cells, may be subsequently quick frozen. |
| format | Patent |
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The quenching ensures most of the metabolic processes within the cells are stopped. The separation device may be a filtration means and the separation and quenching steps may be achieved with a chilled wash solution. 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The quenching ensures most of the metabolic processes within the cells are stopped. The separation device may be a filtration means and the separation and quenching steps may be achieved with a chilled wash solution. The separated cells, which may be animal or insect cells, may be subsequently quick frozen.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Cell suspension separation method |
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