Device for the isolation of nucleic acids in a sealed environment

A device for the isolation of nucleic acid in a sample is provided, comprising an entry port for adding the sample to be analysed to the device, a compartment where the nucleic acid is liberated from the sample, a compartment where the nucleic acid is purified and a compartment where the nucleic aci...

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Bibliographische Detailangaben
Hauptverfasser:	HOLGER KLAPPROTH, ROMNEY JOHN HANNING JACKSON
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A device for the isolation of nucleic acid in a sample is provided, comprising an entry port for adding the sample to be analysed to the device, a compartment where the nucleic acid is liberated from the sample, a compartment where the nucleic acid is purified and a compartment where the nucleic acid is concentrated. The device also comprises a removable container for the isolated nucleic acids. The nucleic acids may then be used in amplification reactions. In a preferred embodiment, the nucleic acid is released from cells and is bound to species-specific or sequence-specific particles. Methods of use of the device are also provided. The device may be used for the purification of microbial nucleic acids in order to detect the contamination of foodstuffs.