Tissue culture using human spleen cells, especially for interferon production

A tissue culture medium characterized in that it comprises the cells originating from human spleens under a form ready for cultivation and a support of culture adequate for the growth of these human spleen cells, preferentially containing seric proteins, still preferentially containing at least in part purified serum or serum treated in a known way to render this support of culture apyrogenic and atoxic. The medium may be used in a method of culture of lymphoblastoid cell lines which are used for the production of interferon. The cells are also used to produce gamma-globulins.