Sistema y método para medir sulfito en muestras alimentarias mediante biosensor amperométrico y uso de dicho biosensor
Sistema para medir sulfito en muestras alimentarias mediante biosensor; método para la determinación de sulfito en muestras alimentarias utilizando el citado biosensor; y uso del citado biosensor para medir los valores de sulfito en muestras alimentarias El sistema utiliza un biosensor amperométrico...
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| creator | ARANTZAMENDI EGIGUREN, Alai GONZALEZ URQUIDI, Irune JAUREGUIBEITIA CAYROLS, Arrate MAZA DEL RIO, Sonia SALLERES ALONSO, Sandra GONZALEZ RIOJA, Roberto |
| description | Sistema para medir sulfito en muestras alimentarias mediante biosensor; método para la determinación de sulfito en muestras alimentarias utilizando el citado biosensor; y uso del citado biosensor para medir los valores de sulfito en muestras alimentarias El sistema utiliza un biosensor amperométrico que comprende un sistema bi-enzimático; un electrodo de trabajo; un contra-electrodo; y un electrodo de referencia El sistema bi-enzimático está compuesto por las enzimas sulfito oxidasa humana y peroxidasa; y.la inmovilización de las enzimas en el electrodo de trabajo se lleva a cabo mediante simple retención física. La mezcla de las enzimas se mezcla a su vez con un mediador químico. El método comprende las fases de aplicación de una muestra al biosensor amperométrico; cambio de corriente eléctrica al entrar en contacto con el analito a detectar; y medir amperométricamente dicha corriente eléctrica en soluciones con agitación continua, utilizando un potencial constante.
The invention relates to a system for measuring sulphite in food samples using a biosensor, a method for determining sulphite in food samples using said biosensor, and the use of said biosensor for measuring the sulphite values in food samples. The system uses an amperometric biosensor comprising a bi-enzymatic system, a working electrode, a counter electrode and a reference electrode. The bi-enzymatic system is composed of the human sulphite oxidase and peroxidase enzymes, and the immobilisation of the enzymes in the working electrode is carried out via simple physical retention. The mixture of the enzymes is mixed with a chemical mediator. The method comprises the following phases: applying a sample to the amperometric biosensor; changing the electrical current upon coming into contact with the analyte to be detected; and amperometrically measuring said electrical current in solutions with continuous agitation, using a constant potential. |
| format | Patent |
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The invention relates to a system for measuring sulphite in food samples using a biosensor, a method for determining sulphite in food samples using said biosensor, and the use of said biosensor for measuring the sulphite values in food samples. The system uses an amperometric biosensor comprising a bi-enzymatic system, a working electrode, a counter electrode and a reference electrode. The bi-enzymatic system is composed of the human sulphite oxidase and peroxidase enzymes, and the immobilisation of the enzymes in the working electrode is carried out via simple physical retention. The mixture of the enzymes is mixed with a chemical mediator. The method comprises the following phases: applying a sample to the amperometric biosensor; changing the electrical current upon coming into contact with the analyte to be detected; and amperometrically measuring said electrical current in solutions with continuous agitation, using a constant potential.</description><language>spa</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210406&DB=EPODOC&CC=ES&NR=2817053T3$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210406&DB=EPODOC&CC=ES&NR=2817053T3$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ARANTZAMENDI EGIGUREN, Alai</creatorcontrib><creatorcontrib>GONZALEZ URQUIDI, Irune</creatorcontrib><creatorcontrib>JAUREGUIBEITIA CAYROLS, Arrate</creatorcontrib><creatorcontrib>MAZA DEL RIO, Sonia</creatorcontrib><creatorcontrib>SALLERES ALONSO, Sandra</creatorcontrib><creatorcontrib>GONZALEZ RIOJA, Roberto</creatorcontrib><title>Sistema y método para medir sulfito en muestras alimentarias mediante biosensor amperométrico y uso de dicho biosensor</title><description>Sistema para medir sulfito en muestras alimentarias mediante biosensor; método para la determinación de sulfito en muestras alimentarias utilizando el citado biosensor; y uso del citado biosensor para medir los valores de sulfito en muestras alimentarias El sistema utiliza un biosensor amperométrico que comprende un sistema bi-enzimático; un electrodo de trabajo; un contra-electrodo; y un electrodo de referencia El sistema bi-enzimático está compuesto por las enzimas sulfito oxidasa humana y peroxidasa; y.la inmovilización de las enzimas en el electrodo de trabajo se lleva a cabo mediante simple retención física. La mezcla de las enzimas se mezcla a su vez con un mediador químico. El método comprende las fases de aplicación de una muestra al biosensor amperométrico; cambio de corriente eléctrica al entrar en contacto con el analito a detectar; y medir amperométricamente dicha corriente eléctrica en soluciones con agitación continua, utilizando un potencial constante.
The invention relates to a system for measuring sulphite in food samples using a biosensor, a method for determining sulphite in food samples using said biosensor, and the use of said biosensor for measuring the sulphite values in food samples. The system uses an amperometric biosensor comprising a bi-enzymatic system, a working electrode, a counter electrode and a reference electrode. The bi-enzymatic system is composed of the human sulphite oxidase and peroxidase enzymes, and the immobilisation of the enzymes in the working electrode is carried out via simple physical retention. The mixture of the enzymes is mixed with a chemical mediator. The method comprises the following phases: applying a sample to the amperometric biosensor; changing the electrical current upon coming into contact with the analyte to be detected; and amperometrically measuring said electrical current in solutions with continuous agitation, using a constant potential.</description><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNzEEKwkAMheFuXIh6h3gAQS2ie6m4b_cldlIMzEyGSQp6JM_hxWxBcOvq8eDjnxePmtUoIDwhvF8mTiBhRgjkOIMOvmcToAhhILWMCug5UDTMPJ6JYTSCG4tSVMmAIVGWKZa5k7E7qIAjcNzd5eeWxaxHr7T67qJYX6rmfN1QkpY0YUeRrK3q_Wl33B7KpinLf8wH7CNIvA</recordid><startdate>20210406</startdate><enddate>20210406</enddate><creator>ARANTZAMENDI EGIGUREN, Alai</creator><creator>GONZALEZ URQUIDI, Irune</creator><creator>JAUREGUIBEITIA CAYROLS, Arrate</creator><creator>MAZA DEL RIO, Sonia</creator><creator>SALLERES ALONSO, Sandra</creator><creator>GONZALEZ RIOJA, Roberto</creator><scope>EVB</scope></search><sort><creationdate>20210406</creationdate><title>Sistema y método para medir sulfito en muestras alimentarias mediante biosensor amperométrico y uso de dicho biosensor</title><author>ARANTZAMENDI EGIGUREN, Alai ; GONZALEZ URQUIDI, Irune ; JAUREGUIBEITIA CAYROLS, Arrate ; MAZA DEL RIO, Sonia ; SALLERES ALONSO, Sandra ; GONZALEZ RIOJA, Roberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_ES2817053TT33</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>spa</language><creationdate>2021</creationdate><topic>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ARANTZAMENDI EGIGUREN, Alai</creatorcontrib><creatorcontrib>GONZALEZ URQUIDI, Irune</creatorcontrib><creatorcontrib>JAUREGUIBEITIA CAYROLS, Arrate</creatorcontrib><creatorcontrib>MAZA DEL RIO, Sonia</creatorcontrib><creatorcontrib>SALLERES ALONSO, Sandra</creatorcontrib><creatorcontrib>GONZALEZ RIOJA, Roberto</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ARANTZAMENDI EGIGUREN, Alai</au><au>GONZALEZ URQUIDI, Irune</au><au>JAUREGUIBEITIA CAYROLS, Arrate</au><au>MAZA DEL RIO, Sonia</au><au>SALLERES ALONSO, Sandra</au><au>GONZALEZ RIOJA, Roberto</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Sistema y método para medir sulfito en muestras alimentarias mediante biosensor amperométrico y uso de dicho biosensor</title><date>2021-04-06</date><risdate>2021</risdate><abstract>Sistema para medir sulfito en muestras alimentarias mediante biosensor; método para la determinación de sulfito en muestras alimentarias utilizando el citado biosensor; y uso del citado biosensor para medir los valores de sulfito en muestras alimentarias El sistema utiliza un biosensor amperométrico que comprende un sistema bi-enzimático; un electrodo de trabajo; un contra-electrodo; y un electrodo de referencia El sistema bi-enzimático está compuesto por las enzimas sulfito oxidasa humana y peroxidasa; y.la inmovilización de las enzimas en el electrodo de trabajo se lleva a cabo mediante simple retención física. La mezcla de las enzimas se mezcla a su vez con un mediador químico. El método comprende las fases de aplicación de una muestra al biosensor amperométrico; cambio de corriente eléctrica al entrar en contacto con el analito a detectar; y medir amperométricamente dicha corriente eléctrica en soluciones con agitación continua, utilizando un potencial constante.
The invention relates to a system for measuring sulphite in food samples using a biosensor, a method for determining sulphite in food samples using said biosensor, and the use of said biosensor for measuring the sulphite values in food samples. The system uses an amperometric biosensor comprising a bi-enzymatic system, a working electrode, a counter electrode and a reference electrode. The bi-enzymatic system is composed of the human sulphite oxidase and peroxidase enzymes, and the immobilisation of the enzymes in the working electrode is carried out via simple physical retention. The mixture of the enzymes is mixed with a chemical mediator. The method comprises the following phases: applying a sample to the amperometric biosensor; changing the electrical current upon coming into contact with the analyte to be detected; and amperometrically measuring said electrical current in solutions with continuous agitation, using a constant potential.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Sistema y método para medir sulfito en muestras alimentarias mediante biosensor amperométrico y uso de dicho biosensor |
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