Proteínas de unión específica y usos de las mismas

Un anticuerpo aislado o uno de sus fragmentos activos, en donde dicho anticuerpo aislado o fragmento activo del mismo: (a) se une a EGFR de tipo salvaje humano unido a la célula cuando el gen EGFR es amplificado; (b) se une a de2-7 EGFR en un epítopo distinto del péptido de empalme LEEKKGNYVVTDH; (c) no se une a células de tipo salvaje que expresan EGFR endógeno; (d) reconoce un epítopo dentro de la secuencia de residuos 273-501 del EGFR de tipo salvaje humano The invention relates to specific binding members, particularly antibodies and active fragments thereof, which recognize an aberrant post-translationally modified, particularly an aberrant glycosylated form of the EGFR. The binding members, particularly antibodies and fragments thereof, of the invention do not bind to EGFR on normal cells in the absence of amplification of the wild- type gene and are capable of binding the de2-7 EGFR at an epitope which is distinct from the junctional peptide. Antibodies of this type are exemplified by the novel antibody 806 whose VH and VL sequences are illustrated as SEQ ID Nos: 2 and 4 and chimeric antibodies thereof as exemplified by ch806.