USO DE UN LAVADO ALCOHOL-SAL PARA LA RECUPERACION EFICAZ DE MICROBACTERIAS Y ADN MICROBACTERIANO DE SEDIMENTO RESPIRATORIO

LA PRESENTE INVENCION ESTA RELACIONADA CON METODOS PARA CONCENTRAR BACTERIAS A PARTIR DE UNA MUESTRA BIOLOGICA VISCOSA. LOS METODOS CONSISTEN EN AÑADIR A LA MUESTRA UN AGENTE HIDROSOLUBLE REDUCTOR DE LA DENSIDAD CON UNA DENSIDAD DE 0,7 A 0,9 G/ML Y UN PUNTO DE EBULLICION SUPERIOR A 50 (GRADOS) C. LA...

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Hauptverfasser: RASHMI, MEHTA, BACKUS, JOHN, W, KERSCHNER, JOANNE H, KREADER, CAROL
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creator RASHMI, MEHTA
BACKUS, JOHN, W
KERSCHNER, JOANNE H
KREADER, CAROL
description LA PRESENTE INVENCION ESTA RELACIONADA CON METODOS PARA CONCENTRAR BACTERIAS A PARTIR DE UNA MUESTRA BIOLOGICA VISCOSA. LOS METODOS CONSISTEN EN AÑADIR A LA MUESTRA UN AGENTE HIDROSOLUBLE REDUCTOR DE LA DENSIDAD CON UNA DENSIDAD DE 0,7 A 0,9 G/ML Y UN PUNTO DE EBULLICION SUPERIOR A 50 (GRADOS) C. LA INVENCION TAMBIEN ESTA RELACIONADA CON METODOS PARA CONCENTRAR BACTERIAS Y ACIDOS NUCLEICOS BACTERIANOS LIBRES A PARTIR DE UNA MUESTRA BIOLOGICA QUE REQUIERE EL MEZCLADO CON LA MUESTRA DE UN AGENTE REDUCTOR DE LA DENSIDAD Y UNA SAL MONOVALENTE. The present invention relates to methods for concentrating bacteria from a viscous biological sample. The methods involve adding to the sample a water-soluble, density-lowering agent having a density of .7 to .9 g/ml and a boiling point greater than 50 DEG C. The invention also relates to methods for concentrating bacteria and free bacterial nucleic acids from a biological sample that involve mixing with the sample a density-lowering agent and a monovalent salt.
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LOS METODOS CONSISTEN EN AÑADIR A LA MUESTRA UN AGENTE HIDROSOLUBLE REDUCTOR DE LA DENSIDAD CON UNA DENSIDAD DE 0,7 A 0,9 G/ML Y UN PUNTO DE EBULLICION SUPERIOR A 50 (GRADOS) C. LA INVENCION TAMBIEN ESTA RELACIONADA CON METODOS PARA CONCENTRAR BACTERIAS Y ACIDOS NUCLEICOS BACTERIANOS LIBRES A PARTIR DE UNA MUESTRA BIOLOGICA QUE REQUIERE EL MEZCLADO CON LA MUESTRA DE UN AGENTE REDUCTOR DE LA DENSIDAD Y UNA SAL MONOVALENTE. The present invention relates to methods for concentrating bacteria from a viscous biological sample. The methods involve adding to the sample a water-soluble, density-lowering agent having a density of .7 to .9 g/ml and a boiling point greater than 50 DEG C. 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LOS METODOS CONSISTEN EN AÑADIR A LA MUESTRA UN AGENTE HIDROSOLUBLE REDUCTOR DE LA DENSIDAD CON UNA DENSIDAD DE 0,7 A 0,9 G/ML Y UN PUNTO DE EBULLICION SUPERIOR A 50 (GRADOS) C. LA INVENCION TAMBIEN ESTA RELACIONADA CON METODOS PARA CONCENTRAR BACTERIAS Y ACIDOS NUCLEICOS BACTERIANOS LIBRES A PARTIR DE UNA MUESTRA BIOLOGICA QUE REQUIERE EL MEZCLADO CON LA MUESTRA DE UN AGENTE REDUCTOR DE LA DENSIDAD Y UNA SAL MONOVALENTE. The present invention relates to methods for concentrating bacteria from a viscous biological sample. The methods involve adding to the sample a water-soluble, density-lowering agent having a density of .7 to .9 g/ml and a boiling point greater than 50 DEG C. 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LOS METODOS CONSISTEN EN AÑADIR A LA MUESTRA UN AGENTE HIDROSOLUBLE REDUCTOR DE LA DENSIDAD CON UNA DENSIDAD DE 0,7 A 0,9 G/ML Y UN PUNTO DE EBULLICION SUPERIOR A 50 (GRADOS) C. LA INVENCION TAMBIEN ESTA RELACIONADA CON METODOS PARA CONCENTRAR BACTERIAS Y ACIDOS NUCLEICOS BACTERIANOS LIBRES A PARTIR DE UNA MUESTRA BIOLOGICA QUE REQUIERE EL MEZCLADO CON LA MUESTRA DE UN AGENTE REDUCTOR DE LA DENSIDAD Y UNA SAL MONOVALENTE. The present invention relates to methods for concentrating bacteria from a viscous biological sample. The methods involve adding to the sample a water-soluble, density-lowering agent having a density of .7 to .9 g/ml and a boiling point greater than 50 DEG C. The invention also relates to methods for concentrating bacteria and free bacterial nucleic acids from a biological sample that involve mixing with the sample a density-lowering agent and a monovalent salt.</abstract><edition>4</edition><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
title USO DE UN LAVADO ALCOHOL-SAL PARA LA RECUPERACION EFICAZ DE MICROBACTERIAS Y ADN MICROBACTERIANO DE SEDIMENTO RESPIRATORIO
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