VACUNA SUBUNITARIA RECOMBINANTE CONTRA LA ENFERMEDAD HEMORRAGICA VIRICA DE LOS CONEJOS

VACUNA SUBUNITARIA RECOMBINANTE CONTRA LA ENFERMEDAD HEMORRAGICA VIRICA DE LOS CONEJOS. SE DESCRIBE LA PRODUCCION DEL ANTIGENO ESTRUCTURAL (VP60) DEL VIRUS DE LA ENFERMEDAD HEMORRAGICA DE LOS CONEJOS RHDV (AISLADO AST/89) ASI COMO SU UTILIZACION COMO VACUNA CONTRA DICHA ENFERMEDAD. LA PRODUCCION DEL...

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Hauptverfasser: MARIN GOMEZ M. SOLEDAD, MARTIN ALONSO JOSE MANUEL, CASAIS GOYOS ROSA, ROCA RIVEIRO JOSE ANTONIO, PARRA FERNANDEZ FRANCISCO
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creator MARIN GOMEZ M. SOLEDAD
MARTIN ALONSO JOSE MANUEL
CASAIS GOYOS ROSA
ROCA RIVEIRO JOSE ANTONIO
PARRA FERNANDEZ FRANCISCO
description VACUNA SUBUNITARIA RECOMBINANTE CONTRA LA ENFERMEDAD HEMORRAGICA VIRICA DE LOS CONEJOS. SE DESCRIBE LA PRODUCCION DEL ANTIGENO ESTRUCTURAL (VP60) DEL VIRUS DE LA ENFERMEDAD HEMORRAGICA DE LOS CONEJOS RHDV (AISLADO AST/89) ASI COMO SU UTILIZACION COMO VACUNA CONTRA DICHA ENFERMEDAD. LA PRODUCCION DEL ANTIGENO Y EL METODO DE VACUNACION COMPRENDEN LAS SIGUIENTES ETAPAS: 1) AISLAMIENTO DEL GEN QUE CODIFICA LA PROTEINA ESTRUCTURAL (VP60) DEL RHDV (AISLADO AST/89); 2) CLONACION DE DICHO GEN EN EL VECTOR DE TRANSFERENCIA DE BACULOVIRUS PACYM1; 3) OBTENCION Y AISLAMIENTO DEL BACULOVIRUS RECOMBINANTE ACVP60; 4) INFECCION DE CELULAS DE INSECTO CON EL BACULOVIRUS RECOMBINANTE ACVP60; 5) OBTENCION Y AISLAMIENTO DE LA PROTEINA VP60 PRODUCIDA POR EL BACULOVIRUS RECOMBINANTE ACVP60; 6) INOCULACION DE LOS CONEJOS CON EL ANTIGENO. Production of the structural antigen (VP60) of the virus of the hemorrhagic disease of the rabbits, RHDV, and its use as a vaccine against said disease. The production of the antigen and the method of vaccination comprise the following stages: a) isolation of the gene that code the structural protein (VP60) of the RHDV; b) cloning of said gene in the baculovirus transfer vector pAcYM1; c) obtention and isolation of the recombinant baculovirus AcVP60; d) infection of Sf9 insect cells with the recombinant baculovirus AcVP60; e) obtention and isolation of the protein VP60 produced by the recombinant baculovirus AcVP60; f) inoculation of the rabbits with the antigen.
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Production of the structural antigen (VP60) of the virus of the hemorrhagic disease of the rabbits, RHDV, and its use as a vaccine against said disease. 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LA PRODUCCION DEL ANTIGENO Y EL METODO DE VACUNACION COMPRENDEN LAS SIGUIENTES ETAPAS: 1) AISLAMIENTO DEL GEN QUE CODIFICA LA PROTEINA ESTRUCTURAL (VP60) DEL RHDV (AISLADO AST/89); 2) CLONACION DE DICHO GEN EN EL VECTOR DE TRANSFERENCIA DE BACULOVIRUS PACYM1; 3) OBTENCION Y AISLAMIENTO DEL BACULOVIRUS RECOMBINANTE ACVP60; 4) INFECCION DE CELULAS DE INSECTO CON EL BACULOVIRUS RECOMBINANTE ACVP60; 5) OBTENCION Y AISLAMIENTO DE LA PROTEINA VP60 PRODUCIDA POR EL BACULOVIRUS RECOMBINANTE ACVP60; 6) INOCULACION DE LOS CONEJOS CON EL ANTIGENO. Production of the structural antigen (VP60) of the virus of the hemorrhagic disease of the rabbits, RHDV, and its use as a vaccine against said disease. The production of the antigen and the method of vaccination comprise the following stages: a) isolation of the gene that code the structural protein (VP60) of the RHDV; b) cloning of said gene in the baculovirus transfer vector pAcYM1; c) obtention and isolation of the recombinant baculovirus AcVP60; d) infection of Sf9 insect cells with the recombinant baculovirus AcVP60; e) obtention and isolation of the protein VP60 produced by the recombinant baculovirus AcVP60; f) inoculation of the rabbits with the antigen.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1996</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyj0KwjAUAOAsDqLe4V2gEKuD60vyaiNNAi8_aykSJ9FCvT9S8ABO3_JtRSmos0eIWWVvE7JFYNLBKevRJwIdfGKEAYF8R-zIoIGeXGDGq9UIxfKKIRhCXDvdQtyLzWN6LvXwcyego6T7ps7vsS7zdK-v-hkptvIiZXtWx9Mf5QslNDAQ</recordid><startdate>19960816</startdate><enddate>19960816</enddate><creator>MARIN GOMEZ M. 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SOLEDAD</creatorcontrib><creatorcontrib>MARTIN ALONSO JOSE MANUEL</creatorcontrib><creatorcontrib>CASAIS GOYOS ROSA</creatorcontrib><creatorcontrib>ROCA RIVEIRO JOSE ANTONIO</creatorcontrib><creatorcontrib>PARRA FERNANDEZ FRANCISCO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MARIN GOMEZ M. SOLEDAD</au><au>MARTIN ALONSO JOSE MANUEL</au><au>CASAIS GOYOS ROSA</au><au>ROCA RIVEIRO JOSE ANTONIO</au><au>PARRA FERNANDEZ FRANCISCO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>VACUNA SUBUNITARIA RECOMBINANTE CONTRA LA ENFERMEDAD HEMORRAGICA VIRICA DE LOS CONEJOS</title><date>1996-08-16</date><risdate>1996</risdate><abstract>VACUNA SUBUNITARIA RECOMBINANTE CONTRA LA ENFERMEDAD HEMORRAGICA VIRICA DE LOS CONEJOS. SE DESCRIBE LA PRODUCCION DEL ANTIGENO ESTRUCTURAL (VP60) DEL VIRUS DE LA ENFERMEDAD HEMORRAGICA DE LOS CONEJOS RHDV (AISLADO AST/89) ASI COMO SU UTILIZACION COMO VACUNA CONTRA DICHA ENFERMEDAD. LA PRODUCCION DEL ANTIGENO Y EL METODO DE VACUNACION COMPRENDEN LAS SIGUIENTES ETAPAS: 1) AISLAMIENTO DEL GEN QUE CODIFICA LA PROTEINA ESTRUCTURAL (VP60) DEL RHDV (AISLADO AST/89); 2) CLONACION DE DICHO GEN EN EL VECTOR DE TRANSFERENCIA DE BACULOVIRUS PACYM1; 3) OBTENCION Y AISLAMIENTO DEL BACULOVIRUS RECOMBINANTE ACVP60; 4) INFECCION DE CELULAS DE INSECTO CON EL BACULOVIRUS RECOMBINANTE ACVP60; 5) OBTENCION Y AISLAMIENTO DE LA PROTEINA VP60 PRODUCIDA POR EL BACULOVIRUS RECOMBINANTE ACVP60; 6) INOCULACION DE LOS CONEJOS CON EL ANTIGENO. Production of the structural antigen (VP60) of the virus of the hemorrhagic disease of the rabbits, RHDV, and its use as a vaccine against said disease. The production of the antigen and the method of vaccination comprise the following stages: a) isolation of the gene that code the structural protein (VP60) of the RHDV; b) cloning of said gene in the baculovirus transfer vector pAcYM1; c) obtention and isolation of the recombinant baculovirus AcVP60; d) infection of Sf9 insect cells with the recombinant baculovirus AcVP60; e) obtention and isolation of the protein VP60 produced by the recombinant baculovirus AcVP60; f) inoculation of the rabbits with the antigen.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title VACUNA SUBUNITARIA RECOMBINANTE CONTRA LA ENFERMEDAD HEMORRAGICA VIRICA DE LOS CONEJOS
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