PROCEDIMIENTO PARA PRODUCIR PROTEINAS MEDIANTE ADN RECOMBINANTE
LA PRESENTE INVENCION PREVEE UN METODO PARA LA PRODUCCION DE FRAGMENTOS FV QUE COMPRENDE: TRANSFORMACION DE UNA CELULA EUCARIOTICA CON UN VECTOR DE EXPRESION AUCARIOTICO QUE COMPRENDE UN OPERON CON UNA SECUENCIA DE DNA QUE CODIFICA PARA UN DOMINIO VARIABLE SOLAMENTE DE UN ANTICUERPO DE CADENA LIGERA...
Gespeichert in:
Hauptverfasser: | , , , , |
---|---|
Format: | Patent |
Sprache: | spa |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | |
container_start_page | |
container_title | |
container_volume | |
creator | YARRANTON, GEOFFREY THOMAS BODMER, MARK WILLIAM OWENS, RAYMOND JOHN WINTER, GREGORY PAUL RIECHMANN, LUTZ |
description | LA PRESENTE INVENCION PREVEE UN METODO PARA LA PRODUCCION DE FRAGMENTOS FV QUE COMPRENDE: TRANSFORMACION DE UNA CELULA EUCARIOTICA CON UN VECTOR DE EXPRESION AUCARIOTICO QUE COMPRENDE UN OPERON CON UNA SECUENCIA DE DNA QUE CODIFICA PARA UN DOMINIO VARIABLE SOLAMENTE DE UN ANTICUERPO DE CADENA LIGERA O PESADA. CULTIVO DE LA CELULA HUESPED TRANSFORMADA BAJO CONDICIONES QUE CAUSAN LA PROTEINA CODIFICADA POR LA SECUENCIA DE DNA QUE SE VA A SINTETIZAR, Y RECOLECCION DE LA PROTEINA SINTETIZADA. FIG.1.
The present invention provides a method for producing Fv fragments comprising: transforming a eukaryotic host cell with a eukaryotic expression vector comprising an operon having a DNA sequence which encodes the variable domain only of an antibody light or heavy chain, culturing the transformed host cell under conditions which cause the protein encoded by the DNA sequence to be synthesised; and collecting the synthesised protein. |
format | Patent |
fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_ES2070172TT3</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ES2070172TT3</sourcerecordid><originalsourceid>FETCH-epo_espacenet_ES2070172TT33</originalsourceid><addsrcrecordid>eNrjZLAPCPJ3dnXx9PV09QvxVwhwDHJUAAq5hDp7BoEYIa6efo7BCr5AJY5-Ia4Kji5-CkGuzv6-TkBxoAAPA2taYk5xKi-U5mZQdHMNcfbQTS3Ij08tLkhMTs1LLYl3DTYyMDcwNDcKCTE2JkYNAKM4KvI</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>PROCEDIMIENTO PARA PRODUCIR PROTEINAS MEDIANTE ADN RECOMBINANTE</title><source>esp@cenet</source><creator>YARRANTON, GEOFFREY THOMAS ; BODMER, MARK WILLIAM ; OWENS, RAYMOND JOHN ; WINTER, GREGORY PAUL ; RIECHMANN, LUTZ</creator><creatorcontrib>YARRANTON, GEOFFREY THOMAS ; BODMER, MARK WILLIAM ; OWENS, RAYMOND JOHN ; WINTER, GREGORY PAUL ; RIECHMANN, LUTZ</creatorcontrib><description>LA PRESENTE INVENCION PREVEE UN METODO PARA LA PRODUCCION DE FRAGMENTOS FV QUE COMPRENDE: TRANSFORMACION DE UNA CELULA EUCARIOTICA CON UN VECTOR DE EXPRESION AUCARIOTICO QUE COMPRENDE UN OPERON CON UNA SECUENCIA DE DNA QUE CODIFICA PARA UN DOMINIO VARIABLE SOLAMENTE DE UN ANTICUERPO DE CADENA LIGERA O PESADA. CULTIVO DE LA CELULA HUESPED TRANSFORMADA BAJO CONDICIONES QUE CAUSAN LA PROTEINA CODIFICADA POR LA SECUENCIA DE DNA QUE SE VA A SINTETIZAR, Y RECOLECCION DE LA PROTEINA SINTETIZADA. FIG.1.
The present invention provides a method for producing Fv fragments comprising: transforming a eukaryotic host cell with a eukaryotic expression vector comprising an operon having a DNA sequence which encodes the variable domain only of an antibody light or heavy chain, culturing the transformed host cell under conditions which cause the protein encoded by the DNA sequence to be synthesised; and collecting the synthesised protein.</description><edition>4</edition><language>spa</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; HUMAN NECESSITIES ; HYGIENE ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>1995</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19950601&DB=EPODOC&CC=ES&NR=2070172T3$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19950601&DB=EPODOC&CC=ES&NR=2070172T3$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YARRANTON, GEOFFREY THOMAS</creatorcontrib><creatorcontrib>BODMER, MARK WILLIAM</creatorcontrib><creatorcontrib>OWENS, RAYMOND JOHN</creatorcontrib><creatorcontrib>WINTER, GREGORY PAUL</creatorcontrib><creatorcontrib>RIECHMANN, LUTZ</creatorcontrib><title>PROCEDIMIENTO PARA PRODUCIR PROTEINAS MEDIANTE ADN RECOMBINANTE</title><description>LA PRESENTE INVENCION PREVEE UN METODO PARA LA PRODUCCION DE FRAGMENTOS FV QUE COMPRENDE: TRANSFORMACION DE UNA CELULA EUCARIOTICA CON UN VECTOR DE EXPRESION AUCARIOTICO QUE COMPRENDE UN OPERON CON UNA SECUENCIA DE DNA QUE CODIFICA PARA UN DOMINIO VARIABLE SOLAMENTE DE UN ANTICUERPO DE CADENA LIGERA O PESADA. CULTIVO DE LA CELULA HUESPED TRANSFORMADA BAJO CONDICIONES QUE CAUSAN LA PROTEINA CODIFICADA POR LA SECUENCIA DE DNA QUE SE VA A SINTETIZAR, Y RECOLECCION DE LA PROTEINA SINTETIZADA. FIG.1.
The present invention provides a method for producing Fv fragments comprising: transforming a eukaryotic host cell with a eukaryotic expression vector comprising an operon having a DNA sequence which encodes the variable domain only of an antibody light or heavy chain, culturing the transformed host cell under conditions which cause the protein encoded by the DNA sequence to be synthesised; and collecting the synthesised protein.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1995</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLAPCPJ3dnXx9PV09QvxVwhwDHJUAAq5hDp7BoEYIa6efo7BCr5AJY5-Ia4Kji5-CkGuzv6-TkBxoAAPA2taYk5xKi-U5mZQdHMNcfbQTS3Ij08tLkhMTs1LLYl3DTYyMDcwNDcKCTE2JkYNAKM4KvI</recordid><startdate>19950601</startdate><enddate>19950601</enddate><creator>YARRANTON, GEOFFREY THOMAS</creator><creator>BODMER, MARK WILLIAM</creator><creator>OWENS, RAYMOND JOHN</creator><creator>WINTER, GREGORY PAUL</creator><creator>RIECHMANN, LUTZ</creator><scope>EVB</scope></search><sort><creationdate>19950601</creationdate><title>PROCEDIMIENTO PARA PRODUCIR PROTEINAS MEDIANTE ADN RECOMBINANTE</title><author>YARRANTON, GEOFFREY THOMAS ; BODMER, MARK WILLIAM ; OWENS, RAYMOND JOHN ; WINTER, GREGORY PAUL ; RIECHMANN, LUTZ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_ES2070172TT33</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>spa</language><creationdate>1995</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YARRANTON, GEOFFREY THOMAS</creatorcontrib><creatorcontrib>BODMER, MARK WILLIAM</creatorcontrib><creatorcontrib>OWENS, RAYMOND JOHN</creatorcontrib><creatorcontrib>WINTER, GREGORY PAUL</creatorcontrib><creatorcontrib>RIECHMANN, LUTZ</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YARRANTON, GEOFFREY THOMAS</au><au>BODMER, MARK WILLIAM</au><au>OWENS, RAYMOND JOHN</au><au>WINTER, GREGORY PAUL</au><au>RIECHMANN, LUTZ</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PROCEDIMIENTO PARA PRODUCIR PROTEINAS MEDIANTE ADN RECOMBINANTE</title><date>1995-06-01</date><risdate>1995</risdate><abstract>LA PRESENTE INVENCION PREVEE UN METODO PARA LA PRODUCCION DE FRAGMENTOS FV QUE COMPRENDE: TRANSFORMACION DE UNA CELULA EUCARIOTICA CON UN VECTOR DE EXPRESION AUCARIOTICO QUE COMPRENDE UN OPERON CON UNA SECUENCIA DE DNA QUE CODIFICA PARA UN DOMINIO VARIABLE SOLAMENTE DE UN ANTICUERPO DE CADENA LIGERA O PESADA. CULTIVO DE LA CELULA HUESPED TRANSFORMADA BAJO CONDICIONES QUE CAUSAN LA PROTEINA CODIFICADA POR LA SECUENCIA DE DNA QUE SE VA A SINTETIZAR, Y RECOLECCION DE LA PROTEINA SINTETIZADA. FIG.1.
The present invention provides a method for producing Fv fragments comprising: transforming a eukaryotic host cell with a eukaryotic expression vector comprising an operon having a DNA sequence which encodes the variable domain only of an antibody light or heavy chain, culturing the transformed host cell under conditions which cause the protein encoded by the DNA sequence to be synthesised; and collecting the synthesised protein.</abstract><edition>4</edition><oa>free_for_read</oa></addata></record> |
fulltext | fulltext_linktorsrc |
identifier | |
ispartof | |
issn | |
language | spa |
recordid | cdi_epo_espacenet_ES2070172TT3 |
source | esp@cenet |
subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
title | PROCEDIMIENTO PARA PRODUCIR PROTEINAS MEDIANTE ADN RECOMBINANTE |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T18%3A10%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=YARRANTON,%20GEOFFREY%20THOMAS&rft.date=1995-06-01&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EES2070172TT3%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |