AN IMPROVED METHOD FOR SEPARATION OF LOW MOLECULAR WEIGHT PROTEINS

The present invention provides the method for the separation of at least more than two Low molecular weight (LMWs) impurities present in the protein mixture comprising fusion protein CTL4-IgG1 by using Capillary Electrophoresis-Sodium dodecyl sulfate (CE-SDS) wherein the loading amount of protein mi...

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Hauptverfasser: KARDANI, Jaykumar Rameshchandra, SHAH, Aakansha, UPADHYAY, Roshan Ganeshlal
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creator KARDANI, Jaykumar Rameshchandra
SHAH, Aakansha
UPADHYAY, Roshan Ganeshlal
description The present invention provides the method for the separation of at least more than two Low molecular weight (LMWs) impurities present in the protein mixture comprising fusion protein CTL4-IgG1 by using Capillary Electrophoresis-Sodium dodecyl sulfate (CE-SDS) wherein the loading amount of protein mixture is more than 270 µg. The invention further provides a ratio of protein to sodium dodecyl sulfate is 2.7:1 or higher.
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subjects CHEMISTRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
TESTING
title AN IMPROVED METHOD FOR SEPARATION OF LOW MOLECULAR WEIGHT PROTEINS
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