CATION EXCHANGE FOR SPERM-ASSOCIATED DNA PURIFICATION

Provided herein are methods, reagents, and kits for the isolation of sperm cells from other cell types and the purification of sperm-associated DNA from a complex sample. In particular, methods, reagents, and kits are provided for the separation of intact sperm cells from epithelial cells via bindin...

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Hauptverfasser: MCLAREN, Robert Stewart, ZENG, Zhiyang, CAO, Jian, ZHOU, Wenhui, COURTNEY, Nicholas A
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creator MCLAREN, Robert Stewart
ZENG, Zhiyang
CAO, Jian
ZHOU, Wenhui
COURTNEY, Nicholas A
description Provided herein are methods, reagents, and kits for the isolation of sperm cells from other cell types and the purification of sperm-associated DNA from a complex sample. In particular, methods, reagents, and kits are provided for the separation of intact sperm cells from epithelial cells via binding of sperm cells to a cation exchange resin, and the extraction of sperm-associated DNA therefrom.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title CATION EXCHANGE FOR SPERM-ASSOCIATED DNA PURIFICATION
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