METHOD FOR PRODUCING BACTERIOPHAGES
The invention relates to a method for producing bacteriophages, which comprises: providing at least one cell culture chamber; providing a first vessel containing a culture medium in a first closed-circuit connection with said at least one cell culture chamber, the culture medium comprising at least...
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Format: | Patent |
Sprache: | eng ; fre ; ger |
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Zusammenfassung: | The invention relates to a method for producing bacteriophages, which comprises: providing at least one cell culture chamber; providing a first vessel containing a culture medium in a first closed-circuit connection with said at least one cell culture chamber, the culture medium comprising at least one strain of phages and at least one strain of bacteria; transferring the culture medium from the first vessel via the first closed-circuit connection into said at least one cell culture chamber in order to deposit the culture medium as a culture layer in said at least one culture chamber; incubating the culture medium so as to propagate phages; providing a container in a second closed-circuit connection with said at least one cell culture chamber, the second closed-circuit connection comprising a filtration system between said at least one cell culture chamber and the container; and transferring the culture medium comprising the propagated phages from said at least one culture chamber to the second closed-circuit connection and purifying said propagated phages via the filtration system, the culture medium being in liquid form during the transfer from said at least one culture chamber to the second closed-circuit connection and during the purification. |
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