MULTIPLE FEATURE INTEGRATION WITH NEXT-GENERATION THREE-DIMENSIONAL IN SITU SEQUENCING

Provided herein are devices, methods, and systems for multiple feature integration with next-generation three-dimensional in situ sequencing of nucleic acids in cells in intact tissue. Biological samples contain many distinct types of molecular, cellular, anatomical, and experimental features. The d...

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Bibliographische Detailangaben
Hauptverfasser: RICHMAN, Ethan B, DEISSEROTH, Karl A
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:Provided herein are devices, methods, and systems for multiple feature integration with next-generation three-dimensional in situ sequencing of nucleic acids in cells in intact tissue. Biological samples contain many distinct types of molecular, cellular, anatomical, and experimental features. The disclosed methods allow simultaneous interrogation of multiple distinct features of a biological sample, including RNA features, anatomical features, exogenous barcodes, or other arbitrary experimental features such as in vivo measurements, which can be combined into single experimental readouts with next-generation in situ sequencing.