A METHOD DIFFERENTIATING BENIGN AND MALIGNANT TYROID NODULES

The method of differentiating benign and malignant thyroid nodules in tumor biopsy material classified according to the Bethesda system includes the steps of RNA isolation and determining the expression level of a set of genes, which includes: AMOT, C3, CAMK2N1, CFI, DCSTAMP, DIO1, DUSP5, EGR1, EMP2...

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Hauptverfasser:	Jarzab, Barbara, Rusinek, Dagmara, Jarzab, Michal, Student, Sebastian, Oczko-Wojciechowska, Malgorzata, Slowinska-Klencka, Dorota, Fujarewicz, Krzysztof, Wilk, Agata, Pluciennik, Alicja, Tyszkiewicz, Tomasz, Lakomiec, Krzysztof, Krajewska, Jolanta, Tarnawski, Rafal, Zebracka, Jadwiga
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The method of differentiating benign and malignant thyroid nodules in tumor biopsy material classified according to the Bethesda system includes the steps of RNA isolation and determining the expression level of a set of genes, which includes: AMOT, C3, CAMK2N1, CFI, DCSTAMP, DIO1, DUSP5, EGR1, EMP2, FAM20A, FAXC, FLRT3, HBG1, IGFBP6, IGSF1, ITGA2, KCNQ3, MET, METTL7B, MPZL2, NOD1, OLR1, PDE5A, PSD3, SDC4, SLC26A4, SLC26A7, SLC5A8, SLPI, TM4SF1, TPO, and TUSC3. Normalization of the expression of the 32 genes in this set is carried out under the endogenous control of at least four normalizing genes among: EIF3A (EIF3S10), HADHA, UBE2D2, EIF5, PPIE, and RPLP0. To differentiate thyroid nodules, a classifier algorithm is used, which is trained on gene expression data correlated with the nature of the thyroid nodule, especially benign and malignant thyroid nodules, including papillary and follicular thyroid carcinoma.