ADVANCED DUMBBELL PCR FOR ISOMIR DETECTION

The present invention relates to a method for the detection of small non-coding RNAs in a sample using sequence-specific structured adapters for the ligation by a double stranded RNA ligase with subsequent cDNA production and quantification. The present invention further relates to a kit for carryin...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: HOROS, Rastislav, STEINKRAUS, Bruno
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:The present invention relates to a method for the detection of small non-coding RNAs in a sample using sequence-specific structured adapters for the ligation by a double stranded RNA ligase with subsequent cDNA production and quantification. The present invention further relates to a kit for carrying out this method.