PROCESS FOR CONSTRUCTION OF A SINGLE VECTOR COMPRISING CAS9 AND SGRNA FOR MYCOBACTERIAL GENOME MODIFICATIONS

The present invention relates to a process for constructing a single vector comprising cas9 and sgRNA ("all-in-one" vector) for mycobacterial genome modifications, comprising the steps of (a) providing the expression cassette of cas9 and the tracrRNA expression cassette with codons optimiz...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	KANNO, Alex Issamu, MORAES, Luana, LEITE, Luciana Cezar De Cerqueira
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page
container_issue
container_start_page
container_title
container_volume
creator	KANNO, Alex Issamu MORAES, Luana LEITE, Luciana Cezar De Cerqueira
description	The present invention relates to a process for constructing a single vector comprising cas9 and sgRNA ("all-in-one" vector) for mycobacterial genome modifications, comprising the steps of (a) providing the expression cassette of cas9 and the tracrRNA expression cassette with codons optimized for expression in mycobacteria and clone into a cloning vector; (b) sequentially cloning the sequences optimized in step (a) into a mycobacterial bridge vector; (c) selecting targeting sequences (crRNAs) specific to the gene of interest; (d) inserting the crRNAs selected in step (c) into the mycobacterial expression vector, upstream of the tracrRNA sequence, at position +1 downstream of an inducible promoter for the construction of the sgRNA cassette; and (e) adding at the end of each expression cassette a transcriptional terminator.
format	Patent
fullrecord	<record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_EP4265722A2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>EP4265722A2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_EP4265722A23</originalsourceid><addsrcrecordid>eNqNjE0KwjAQhbtxIeod5gJu4h8ux-mkBkymZKLgqhSJq6KFen-kxQO4evC-97150dVRiFXBSgSSoCleKTkJIBYQ1IXqwnBjShP3dXRjB4R6BAwlaBUDTra_k5yQEkeHF6g4iGfwUjrrCMdLXRazZ9sNefXLRQGWE53XuX83eejbR37lT8P11ux3B2PQbP6YfAGRFzZU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>PROCESS FOR CONSTRUCTION OF A SINGLE VECTOR COMPRISING CAS9 AND SGRNA FOR MYCOBACTERIAL GENOME MODIFICATIONS</title><source>esp@cenet</source><creator>KANNO, Alex Issamu ; MORAES, Luana ; LEITE, Luciana Cezar De Cerqueira</creator><creatorcontrib>KANNO, Alex Issamu ; MORAES, Luana ; LEITE, Luciana Cezar De Cerqueira</creatorcontrib><description>The present invention relates to a process for constructing a single vector comprising cas9 and sgRNA ("all-in-one" vector) for mycobacterial genome modifications, comprising the steps of (a) providing the expression cassette of cas9 and the tracrRNA expression cassette with codons optimized for expression in mycobacteria and clone into a cloning vector; (b) sequentially cloning the sequences optimized in step (a) into a mycobacterial bridge vector; (c) selecting targeting sequences (crRNAs) specific to the gene of interest; (d) inserting the crRNAs selected in step (c) into the mycobacterial expression vector, upstream of the tracrRNA sequence, at position +1 downstream of an inducible promoter for the construction of the sgRNA cassette; and (e) adding at the end of each expression cassette a transcriptional terminator.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231025&DB=EPODOC&CC=EP&NR=4265722A2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231025&DB=EPODOC&CC=EP&NR=4265722A2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KANNO, Alex Issamu</creatorcontrib><creatorcontrib>MORAES, Luana</creatorcontrib><creatorcontrib>LEITE, Luciana Cezar De Cerqueira</creatorcontrib><title>PROCESS FOR CONSTRUCTION OF A SINGLE VECTOR COMPRISING CAS9 AND SGRNA FOR MYCOBACTERIAL GENOME MODIFICATIONS</title><description>The present invention relates to a process for constructing a single vector comprising cas9 and sgRNA ("all-in-one" vector) for mycobacterial genome modifications, comprising the steps of (a) providing the expression cassette of cas9 and the tracrRNA expression cassette with codons optimized for expression in mycobacteria and clone into a cloning vector; (b) sequentially cloning the sequences optimized in step (a) into a mycobacterial bridge vector; (c) selecting targeting sequences (crRNAs) specific to the gene of interest; (d) inserting the crRNAs selected in step (c) into the mycobacterial expression vector, upstream of the tracrRNA sequence, at position +1 downstream of an inducible promoter for the construction of the sgRNA cassette; and (e) adding at the end of each expression cassette a transcriptional terminator.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjE0KwjAQhbtxIeod5gJu4h8ux-mkBkymZKLgqhSJq6KFen-kxQO4evC-97150dVRiFXBSgSSoCleKTkJIBYQ1IXqwnBjShP3dXRjB4R6BAwlaBUDTra_k5yQEkeHF6g4iGfwUjrrCMdLXRazZ9sNefXLRQGWE53XuX83eejbR37lT8P11ux3B2PQbP6YfAGRFzZU</recordid><startdate>20231025</startdate><enddate>20231025</enddate><creator>KANNO, Alex Issamu</creator><creator>MORAES, Luana</creator><creator>LEITE, Luciana Cezar De Cerqueira</creator><scope>EVB</scope></search><sort><creationdate>20231025</creationdate><title>PROCESS FOR CONSTRUCTION OF A SINGLE VECTOR COMPRISING CAS9 AND SGRNA FOR MYCOBACTERIAL GENOME MODIFICATIONS</title><author>KANNO, Alex Issamu ; MORAES, Luana ; LEITE, Luciana Cezar De Cerqueira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP4265722A23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KANNO, Alex Issamu</creatorcontrib><creatorcontrib>MORAES, Luana</creatorcontrib><creatorcontrib>LEITE, Luciana Cezar De Cerqueira</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KANNO, Alex Issamu</au><au>MORAES, Luana</au><au>LEITE, Luciana Cezar De Cerqueira</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PROCESS FOR CONSTRUCTION OF A SINGLE VECTOR COMPRISING CAS9 AND SGRNA FOR MYCOBACTERIAL GENOME MODIFICATIONS</title><date>2023-10-25</date><risdate>2023</risdate><abstract>The present invention relates to a process for constructing a single vector comprising cas9 and sgRNA ("all-in-one" vector) for mycobacterial genome modifications, comprising the steps of (a) providing the expression cassette of cas9 and the tracrRNA expression cassette with codons optimized for expression in mycobacteria and clone into a cloning vector; (b) sequentially cloning the sequences optimized in step (a) into a mycobacterial bridge vector; (c) selecting targeting sequences (crRNAs) specific to the gene of interest; (d) inserting the crRNAs selected in step (c) into the mycobacterial expression vector, upstream of the tracrRNA sequence, at position +1 downstream of an inducible promoter for the construction of the sgRNA cassette; and (e) adding at the end of each expression cassette a transcriptional terminator.</abstract><oa>free_for_read</oa></addata></record>
fulltext	fulltext_linktorsrc
identifier
ispartof
issn
language	eng ; fre ; ger
recordid	cdi_epo_espacenet_EP4265722A2
source	esp@cenet
subjects	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
title	PROCESS FOR CONSTRUCTION OF A SINGLE VECTOR COMPRISING CAS9 AND SGRNA FOR MYCOBACTERIAL GENOME MODIFICATIONS
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T22%3A51%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=KANNO,%20Alex%20Issamu&rft.date=2023-10-25&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EEP4265722A2%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true