METHODS OF ASSAYING A BIOLOGICAL CELL
Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can enco...
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| creator | DAILEY, Katrine Elise KIM, John Junyeon SHANSAB, Maryam MAST, Joshua David KUBIT, Matthew Asuka KUBATUR, Shruthi Sreedhar ZHOU, Hongye HU, Zhen TENNEY, John A PARK, Minha PAI, Vincent Haw Tien HIGA, Adrienne T NG, Preston Lock BRIGGS, Jason C ELLEFSON, Arlvin Louis TUNG, Po-Yuan INGRAM, Patrick N MCEWEN, Jason M OLSON, Alexander Gerald |
| description | Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can encode proteins of interest, such as B cell receptor (BCR) heavy and light chain sequences. The expression constructs can be generated individually or in a paired/multiplexed manner, allowing rapid re-expression of individual proteins or protein complexes. |
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Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can encode proteins of interest, such as B cell receptor (BCR) heavy and light chain sequences. 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Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can encode proteins of interest, such as B cell receptor (BCR) heavy and light chain sequences. The expression constructs can be generated individually or in a paired/multiplexed manner, allowing rapid re-expression of individual proteins or protein complexes.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES TESTING |
| title | METHODS OF ASSAYING A BIOLOGICAL CELL |
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