INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING TEMPERATURE SHIFT
The present invention relates to the field of industrial fermentation. In particular, it relates to a method for cultivating a Bacillus host cell comprising the steps of (a) inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein o...
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| creator | DAUB, Andreas WANDREY, Georg Benjamin KLEIN, Tobias GOLABGIR ANBARANI, Aydin MORWEISER, Michael |
| description | The present invention relates to the field of industrial fermentation. In particular, it relates to a method for cultivating a Bacillus host cell comprising the steps of (a) inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein of interest, (b) cultivating for a first cultivation phase the Bacillus host cell in said fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation of the Bacillus host cell comprises the addition of at least one feed solution and wherein the cultivation during the first cultivation phase is carried out at a first temperature, and (c) cultivating for a second cultivation phase the Bacillus host cell culture obtained in step (b) under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation comprises the addition of at least one feed solution and wherein the cultivation during the second cultivation phase is carried out at a second temperature, said second temperature being higher than the first temperature. The invention also provides for a Bacillus host cell culture obtainable by the said method. |
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In particular, it relates to a method for cultivating a Bacillus host cell comprising the steps of (a) inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein of interest, (b) cultivating for a first cultivation phase the Bacillus host cell in said fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation of the Bacillus host cell comprises the addition of at least one feed solution and wherein the cultivation during the first cultivation phase is carried out at a first temperature, and (c) cultivating for a second cultivation phase the Bacillus host cell culture obtained in step (b) under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation comprises the addition of at least one feed solution and wherein the cultivation during the second cultivation phase is carried out at a second temperature, said second temperature being higher than the first temperature. The invention also provides for a Bacillus host cell culture obtainable by the said method.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230607&DB=EPODOC&CC=EP&NR=4189103A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76418</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230607&DB=EPODOC&CC=EP&NR=4189103A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>DAUB, Andreas</creatorcontrib><creatorcontrib>WANDREY, Georg Benjamin</creatorcontrib><creatorcontrib>KLEIN, Tobias</creatorcontrib><creatorcontrib>GOLABGIR ANBARANI, Aydin</creatorcontrib><creatorcontrib>MORWEISER, Michael</creatorcontrib><title>INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING TEMPERATURE SHIFT</title><description>The present invention relates to the field of industrial fermentation. In particular, it relates to a method for cultivating a Bacillus host cell comprising the steps of (a) inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein of interest, (b) cultivating for a first cultivation phase the Bacillus host cell in said fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation of the Bacillus host cell comprises the addition of at least one feed solution and wherein the cultivation during the first cultivation phase is carried out at a first temperature, and (c) cultivating for a second cultivation phase the Bacillus host cell culture obtained in step (b) under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation comprises the addition of at least one feed solution and wherein the cultivation during the second cultivation phase is carried out at a second temperature, said second temperature being higher than the first temperature. The invention also provides for a Bacillus host cell culture obtainable by the said method.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHDx9HMJDQ4J8nT0UXBzDfJ19QtxDPH091MICPJ3dg0OVnDzD1JwcnT29PEJDVYIDfb0c1cIcfUNcA1yDAkNclUI9vB0C-FhYE1LzClO5YXS3AxAs0KcPXRTC_LjU4sLEpNT81JL4l0DTAwtLA0NjB0NjYlQAgBBpSvZ</recordid><startdate>20230607</startdate><enddate>20230607</enddate><creator>DAUB, Andreas</creator><creator>WANDREY, Georg Benjamin</creator><creator>KLEIN, Tobias</creator><creator>GOLABGIR ANBARANI, Aydin</creator><creator>MORWEISER, Michael</creator><scope>EVB</scope></search><sort><creationdate>20230607</creationdate><title>INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING TEMPERATURE SHIFT</title><author>DAUB, Andreas ; WANDREY, Georg Benjamin ; KLEIN, Tobias ; GOLABGIR ANBARANI, Aydin ; MORWEISER, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP4189103A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>DAUB, Andreas</creatorcontrib><creatorcontrib>WANDREY, Georg Benjamin</creatorcontrib><creatorcontrib>KLEIN, Tobias</creatorcontrib><creatorcontrib>GOLABGIR ANBARANI, Aydin</creatorcontrib><creatorcontrib>MORWEISER, Michael</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>DAUB, Andreas</au><au>WANDREY, Georg Benjamin</au><au>KLEIN, Tobias</au><au>GOLABGIR ANBARANI, Aydin</au><au>MORWEISER, Michael</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING TEMPERATURE SHIFT</title><date>2023-06-07</date><risdate>2023</risdate><abstract>The present invention relates to the field of industrial fermentation. In particular, it relates to a method for cultivating a Bacillus host cell comprising the steps of (a) inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein of interest, (b) cultivating for a first cultivation phase the Bacillus host cell in said fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation of the Bacillus host cell comprises the addition of at least one feed solution and wherein the cultivation during the first cultivation phase is carried out at a first temperature, and (c) cultivating for a second cultivation phase the Bacillus host cell culture obtained in step (b) under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation comprises the addition of at least one feed solution and wherein the cultivation during the second cultivation phase is carried out at a second temperature, said second temperature being higher than the first temperature. The invention also provides for a Bacillus host cell culture obtainable by the said method.</abstract><oa>free_for_read</oa></addata></record> |
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| language | eng ; fre ; ger |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING SPIRITS VINEGAR WINE |
| title | INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING TEMPERATURE SHIFT |
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