METHOD OF SPATIAL SEQUENCING OF GENES FROM TISSUE USING PADLOCKS WITH GAPS ON SUBSTRATE

The invention is directed to a method to obtain the spatial location and sequence information of a target sequence in a sample comprising at least one m-RNA strand comprising the stepsa. providing a surface with a plurality of spacer units capable of binding at least one m-RNA strand and with at lea...

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Hauptverfasser: MEYER, Hansueli, PINARD, Robert
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PINARD, Robert
description The invention is directed to a method to obtain the spatial location and sequence information of a target sequence in a sample comprising at least one m-RNA strand comprising the stepsa. providing a surface with a plurality of spacer units capable of binding at least one m-RNA strand and with at least one fiducial markerb. providing a sample comprising at least one m-RNA strand to the surface wherein at least one m-RNA strand of the sample binds to at least one spacer unit creating at least one single stranded oligomerc. taking a first image of the surface to obtain the spatial information of the sample relative to the fiducial markerd. removing sample form surfacee. hybridizing at least one oligonucleotide comprising 50 - 1000 nucleic acids with its 5' and 3' ends to complementary parts of the single stranded oligomer thereby forming a padlock-shaped structure that is ligated to create a single strand circular templatef. multiplying the single strand circular template by a polymerase capable of rolling circle amplification into a plurality of DNA concatemers thereby forming roloniesg. obtaining the sequence information of the roloniesh. linking the spatial information of the sample with the sequence information of the rolonies.
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language eng ; fre ; ger
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title METHOD OF SPATIAL SEQUENCING OF GENES FROM TISSUE USING PADLOCKS WITH GAPS ON SUBSTRATE
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