METABOLOMICS RELATIVE QUANTITATIVE ANALYSIS METHOD BASED ON UPLC/HMRS

METABOLOMICS RELATIVE QUANTITATIVE ANALYSIS METHOD BASED ON UPLC/HMRS

The present invention belongs to the technical field of metabolomics, and relates to a metabolomics relative quantitative analysis method based on UPLC/HRMS. Specifically, the present method mainly comprises the steps of formulating an isotope internal standard mixed solution, a standard curve corre...

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Hauptverfasser: GU, Congshun, XIE, Guigang, REN, Jianhong, GU, Mengnan, XU, Ying, DAI, Zhenli
Format: Patent
Sprache:eng ; fre ; ger
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator GU, Congshun
XIE, Guigang
REN, Jianhong
GU, Mengnan
XU, Ying
DAI, Zhenli
description The present invention belongs to the technical field of metabolomics, and relates to a metabolomics relative quantitative analysis method based on UPLC/HRMS. Specifically, the present method mainly comprises the steps of formulating an isotope internal standard mixed solution, a standard curve correction solution, and a metabolomics sample solution; acquiring raw mass spectrum data of the standard curve correction solution and the metabolomics sample solution; transposing and deconvolving the raw data; identifying and selecting the optimal isotope internal standard; fitting a linear equation and calculating the relative quantitative results of metabolites in the metabolomics sample solution; and completing the structural identification of metabolites and differential metabolites in the metabolomics sample solution. The method can meet both qualitative and quantitative requirements using only a high-resolution mass spectrometry platform; and the quantitative results are accurate, and the accuracy of the qualitative results are higher, having low costs, simple operation, and wide applicability.
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title METABOLOMICS RELATIVE QUANTITATIVE ANALYSIS METHOD BASED ON UPLC/HMRS
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