METHOD COMBINING TARGETED RNA OR C-DNA IN VITRO SEQUENCING USING PADLOCK OLIGONUCLEOTIDES COMPRISING A SPATIAL UNIQUE MOLECULAR IDENTIFIER (SUMI) AND IN SITU SEQUENCING
Microscopy imaging that allow for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention...
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| creator | SPIECKER, Heinrich NEHME, Chris NEIL, Emily ROTHMANN, Thomas HOSONO, Seiyu MEYER, Hansueli PINARD, Robert HINDMAN, Ryan BOSIO, Andreas |
| description | Microscopy imaging that allow for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention describes a method (SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ sequencing and in vitro sequencing of rolonies derived from rolling circle amplification from padlock oligonucleotides targeting portion of RNA or cDNA transcript at a subcellular level with less limitation in the amount of transcripts and the length of the sequence that can be analyzed. Apart from padlocks oligonucleotides, the SUMI-Seq method can also be applied using circular oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results. |
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| language | eng ; fre ; ger |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | METHOD COMBINING TARGETED RNA OR C-DNA IN VITRO SEQUENCING USING PADLOCK OLIGONUCLEOTIDES COMPRISING A SPATIAL UNIQUE MOLECULAR IDENTIFIER (SUMI) AND IN SITU SEQUENCING |
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